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The basic configuration of polarized optical microscope. Copyright: Nikon Corporation.
A schematic representation of the polarization of light waves. Copyright: Nikon Corporation.

Theory of birefringence

Birefringent or doubly-refracting sample has a unique property that it can produce two individual wave components while one wave passes through it, those two components are termed as ordinary and extraordinary waves. [link] is an illustration of a typical construction of Nicol polarizing prism, as we can see, the non-plarized white light are splitted into two ray as it passes through the prism. The one travels out of the prism is called ordinary ray, and the other one is called extraordinary ray. So if we have a birefringent specimen located between the polarizer and analyzer, the initial light will be separated into two waves when it passes though the specimen. After exiting the specimen, the light components become out of phase, but are recombined with constructive and destructive interference when they pass through the analyzer. Now the combined wave will have elliptically or circularly polarized light wave, see [link] , image contrast arises from the interaction of plane-polarized light with a birefringent specimen so some amount of wave will pass through the analyzer and give a bright domain on the specimen.

A schematic representation of a Nicol polarzing prism. Copyright: Nikon Corporation.
A schematic representation of elliptically and circularly polarized light waves. Copyright: Nikon Corporation.

Liquid crystal display

The most common application of LCs are in liquid crystals displays (LCD). [link] is a simple demonstration of how LCD works in digit calculators. There are two crossed polarizers in this system, and liquid crystal (cholesteric spiral pattern) sandwich with positive and negative charging is located between these two polarizers. When the liquid crystal is charged, waves can pass through without changing orientations. When the liquid crystal is out of charge, waves will be rotated 90° as it passes through LCs so it can pass through the second polarizer. There are seven separately charged electrodes in the LCD, so the LCD can exhibit different numbers from 0 to 9 by adjusting the electrodes. For example, when the upper right and lower left electrodes are charged, we can get 2 on the display.

Demonstration of a seven-segment liquid crystal display. Copyright: Nikon Corporation.

Microscope images of liquid crystal phase

The first order retardation plate

The first order retardation plate is frequently utilized to determine the optical sign of a birefringent specimen in polarized light microscopy. The optical sign includes positive and negative. If the ordinary wavefront is faster than the extraordinary wavefront (see [link] ), the specimen displays positive birefringence. Conversly, a negative birefringence will be detected if the ordinary wavefront is slower than the extraordinary wavefront. In addition, the retardation plate is also useful for enhancing contrast in weakly birefringent specimens. [link] shows the effect of first order retardation plate on the contrast of birefringence. The birefringence is so weak that the morphology on the edge of the tissue is hard to image, [link] a. When a first order retardation plate is added, the structure of the cell become all apparent compared with the one without retardation plate, [link] b.

Microscope images of thin section of human tongue, (a) without first order retardation plate and (b) with first order retardation plate. Copyright: Olympus.

Images of liquid crystal phases

[link] shows the images of liquid crystal phases from different specimens. First order retardation plates are utilized in all of these images. Apparent contrasts are detected here in the image which corresponds to the existence of liquid crystal phase within the specimen.

Microscope images in polarized light with a first-order retardation plate inserted between the specimen and analyzer: (a) polyethylene glycol, (b) polycarbonate, and (c) liquid crystalline DNA. Copyright from Nikon.

The effect of rotation of the polarizer

The effect of the angle between horizontal direction and polarizer transmission axis on the appearance of liquid crystal phase may be analyzed. In [link] is shown images of an ascorbic acid ( [link] ) sample under cross polar mode. When the polarizer rotates from 0° to 90°, big variations on the shape of bright domains and domain colors appear due to the change of wave vibrating directions. By rotating the polarizer, we can have a comprehensive understanding of the overall texture.

Cross polarized Microscope images of ascorbic acid specimen with polarizer rotation of (a) 0°, (b) 45°, and (c) 90°. Copyright: Nikon Corporation.
The structure of ascorbic acid.


  • R. Weaver, Am. Lab. , 2003, 35 , 55.
  • F. Massoumian, R. Juskaitis, M. A. Neil, and T. Wilson, J. Microsc. , 2003, 209 , 13.
  • R. Oldenbourg, Nature , 1996, 381 , 811.

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
what school?
biomolecules are e building blocks of every organics and inorganic materials.
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
sciencedirect big data base
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
advantages of NAA
Sai Reply
how I can reaction of mercury?
Sham Reply

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