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Extraction modes

There are two modes in terms of collecting and detecting the components:

  • Off-line extraction.
  • On-line extraction.

Off-line extraction is done by taking the mobile phase out with the extracted components and directing them towards the collection chamber. At this point, supercritical fluid phase is evaporated and released to atmosphere and the components are captured in a solution or a convenient adsorption surface. Then the extracted fragments are processed and prepared for a separation method. This extra manipulation step between extractor and chromatography instrument can cause errors. The on-line method is more sensitive because it directly transfers all extracted materials to a separation unit, mostly a chromatography instrument, without taking them out of the mobile phase. In this extraction/detection type, there is no extra sample preparation after extraction for separation process. This minimizes the errors coming from manipulation steps. Additionally, sample loss does not occur and sensitivity increases.

Applications of sfe

SFE can be applied to a broad range of materials such as polymers, oils and lipids, carbonhydrates, pesticides, organic pollutants, volatile toxins, polyaromatic hydrocarbons, biomolecules, foods, flavors, pharmaceutical metabolites, explosives, and organometallics, etc. Common industrial applications include the pharmaceutical and biochemical industry, the polymer industry, industrial synthesis and extraction, natural product chemistry, and the food industry.

Examples of materials analyzed in environmental applications: oils and fats, pesticides, alkanes, organic pollutants, volatile toxins, herbicides, nicotin, phenanthrene, fatty acids, aromatic surfactants in samples from clay to petroleum waste, from soil to river sediments. In food analyses: caffeine, peroxides, oils, acids, cholesterol, etc. are extracted from samples such as coffee, olive oil, lemon, cereals, wheat, potatoes and dog feed. Through industrial applications, the extracted materials vary from additives to different oligomers, and from petroleum fractions to stabilizers. Samples analyzed are plastics, PVC, paper, wood etc. Drug metabolites, enzymes, steroids are extracted from plasma, urine, serum or animal tissues in biochemical applications.


Supercritical fluid chromatography and supercritical fluid extraction are techniques that take advantage of the unique properties of supercritical fluids. As such, they provide advantages over other related methods in both chromatography and extraction. Sometimes they are used as alternative analytical techniques, while other times they are used as complementary partners for binary systems. Both SFC and SFE demonstrate their versatility through the wide array of applications in many distinct domains in an advantageous way.


  • M. Caude and D. Thiebaut, Practical Supercritical Fluid Chromatography and Extraction , Harwood Academic Publishers, Switzerland (1999).
  • M. D. Luque de Castro, M. Valcarcel, and M. T. Tena, Analytical Supercritical Fluid Extraction , Springer-Verlag, Berlin (1994).
  • D. A. Skoog and J. J. Leary, Principles of Instrumental Analysis , Saunders College Publishing, Philadelphia (1992).
  • L. T. Taylor, Supercritical Fluid Extraction , Wiley-Interscience Publication, New York, (1996).

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
what school?
biomolecules are e building blocks of every organics and inorganic materials.
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
sciencedirect big data base
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
Privacy Information Security Software Version 1.1a
advantages of NAA
Sai Reply
how I can reaction of mercury?
Sham Reply

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