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By the end of this section, you will be able to:
  • Explain the physics behind the operation of microscopes and telescopes
  • Describe the image created by these instruments and calculate their magnifications

Microscopes and telescopes are major instruments that have contributed hugely to our current understanding of the micro- and macroscopic worlds. The invention of these devices led to numerous discoveries in disciplines such as physics, astronomy, and biology, to name a few. In this section, we explain the basic physics that make these instruments work.

Microscopes

Although the eye is marvelous in its ability to see objects large and small, it obviously is limited in the smallest details it can detect. The desire to see beyond what is possible with the naked eye led to the use of optical instruments. We have seen that a simple convex lens can create a magnified image, but it is hard to get large magnification with such a lens. A magnification greater than 5 × is difficult without distorting the image. To get higher magnification, we can combine the simple magnifying glass with one or more additional lenses. In this section, we examine microscopes that enlarge the details that we cannot see with the naked eye.

Microscopes were first developed in the early 1600s by eyeglass makers in The Netherlands and Denmark. The simplest compound microscope    is constructed from two convex lenses ( [link] ). The objective    lens is a convex lens of short focal length (i.e., high power) with typical magnification from 5 × to 100 × . The eyepiece    , also referred to as the ocular, is a convex lens of longer focal length.

The purpose of a microscope is to create magnified images of small objects, and both lenses contribute to the final magnification. Also, the final enlarged image is produced sufficiently far from the observer to be easily viewed, since the eye cannot focus on objects or images that are too close (i.e., closer than the near point of the eye).

Figure shows from left to right: an object with height h, a bi-convex lens labeled objective lens at a distance d subscript o from the object, an inverted image with height h subscript i labeled first image at a distance d subscript i from the objective lens, a bi-convex lens labeled eyepiece at a distance d subscript o prime from the first image and finally the eye of the observer. Rays originate from the top of the object and pass through the objective lens to converge at the top of the inverted image. They travel further and enter the eyepiece, from where they deviate to reach the eye. The back extensions of the deviated rays converge at the tip of a much larger inverted image to the far left of the figure. The height of this image is h subscript i prime and its distance from the eyepiece is d subscript i prime.
A compound microscope is composed of two lenses: an objective and an eyepiece. The objective forms the first image, which is larger than the object. This first image is inside the focal length of the eyepiece and serves as the object for the eyepiece. The eyepiece forms final image that is further magnified.

To see how the microscope in [link] forms an image, consider its two lenses in succession. The object is just beyond the focal length f obj of the objective lens, producing a real, inverted image that is larger than the object. This first image serves as the object for the second lens, or eyepiece. The eyepiece is positioned so that the first image is within its focal length f eye , so that it can further magnify the image. In a sense, it acts as a magnifying glass that magnifies the intermediate image produced by the objective. The image produced by the eyepiece is a magnified virtual image. The final image remains inverted but is farther from the observer than the object, making it easy to view.

The eye views the virtual image created by the eyepiece, which serves as the object for the lens in the eye. The virtual image formed by the eyepiece is well outside the focal length of the eye, so the eye forms a real image on the retina.

Questions & Answers

how does Neisseria cause meningitis
Nyibol Reply
what is microbiologist
Muhammad Reply
what is errata
Muhammad
is the branch of biology that deals with the study of microorganisms.
Ntefuni Reply
What is microbiology
Mercy Reply
studies of microbes
Louisiaste
when we takee the specimen which lumbar,spin,
Ziyad Reply
How bacteria create energy to survive?
Muhamad Reply
Bacteria doesn't produce energy they are dependent upon their substrate in case of lack of nutrients they are able to make spores which helps them to sustain in harsh environments
_Adnan
But not all bacteria make spores, l mean Eukaryotic cells have Mitochondria which acts as powerhouse for them, since bacteria don't have it, what is the substitution for it?
Muhamad
they make spores
Louisiaste
what is sporadic nd endemic, epidemic
Aminu Reply
the significance of food webs for disease transmission
Abreham
food webs brings about an infection as an individual depends on number of diseased foods or carriers dully.
Mark
explain assimilatory nitrate reduction
Esinniobiwa Reply
Assimilatory nitrate reduction is a process that occurs in some microorganisms, such as bacteria and archaea, in which nitrate (NO3-) is reduced to nitrite (NO2-), and then further reduced to ammonia (NH3).
Elkana
This process is called assimilatory nitrate reduction because the nitrogen that is produced is incorporated in the cells of microorganisms where it can be used in the synthesis of amino acids and other nitrogen products
Elkana
Examples of thermophilic organisms
Shu Reply
Give Examples of thermophilic organisms
Shu
advantages of normal Flora to the host
Micheal Reply
Prevent foreign microbes to the host
Abubakar
they provide healthier benefits to their hosts
ayesha
They are friends to host only when Host immune system is strong and become enemies when the host immune system is weakened . very bad relationship!
Mark
what is cell
faisal Reply
cell is the smallest unit of life
Fauziya
cell is the smallest unit of life
Akanni
ok
Innocent
cell is the structural and functional unit of life
Hasan
is the fundamental units of Life
Musa
what are emergency diseases
Micheal Reply
There are nothing like emergency disease but there are some common medical emergency which can occur simultaneously like Bleeding,heart attack,Breathing difficulties,severe pain heart stock.Hope you will get my point .Have a nice day ❣️
_Adnan
define infection ,prevention and control
Innocent
I think infection prevention and control is the avoidance of all things we do that gives out break of infections and promotion of health practices that promote life
Lubega
Heyy Lubega hussein where are u from?
_Adnan
en français
Adama
which site have a normal flora
ESTHER Reply
Many sites of the body have it Skin Nasal cavity Oral cavity Gastro intestinal tract
Safaa
skin
Asiina
skin,Oral,Nasal,GIt
Sadik
How can Commensal can Bacteria change into pathogen?
Sadik
How can Commensal Bacteria change into pathogen?
Sadik
all
Tesfaye
by fussion
Asiina
what are the advantages of normal Flora to the host
Micheal
what are the ways of control and prevention of nosocomial infection in the hospital
Micheal
what is inflammation
Shelly Reply
part of a tissue or an organ being wounded or bruised.
Wilfred
what term is used to name and classify microorganisms?
Micheal Reply
Binomial nomenclature
adeolu
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Source:  OpenStax, University physics volume 3. OpenStax CNX. Nov 04, 2016 Download for free at http://cnx.org/content/col12067/1.4
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