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Segmentation block diagram

segmentation

Green channel isolation

As all images were in RGB format, green channel isolation simply involved taking the green layer of the matrix containing the image, returning a grayscale image based on the green intensity of the original image. Recall that this was done to match the white blood cell stains.

Thresholding

Thresholding was performed on the grayscale images with MATLAB's multithresh function using Otsu's method, separating the image with 5 different thresholds into 6 different levels. The 2nd most exclusive threshold was used to select nuclei, and the 4th most exclusive threshold was used to select cell bodies, returning two rough binary masks.

Cleaning

These rough masks were then processed to remove debris, fill holes, and smooth edges.
  • Debris was removed by deleting all objects with a centroid within a fixed distance from the margins or smaller than a fixed number of pixels. The objects and their centroids were found using MATLAB's regionprops function.
  • Holes were filled using MATLAB's imfill function and imclose function.
  • Since debris overlapping with the cell or nucleus of interest were not removed with the previous debris clearing function, a binary erosion was performed on the mask, the debris clearing function was repeated, and a binary dilation was performed on the mask, using MATLAB's imerode and imdilate functions, respectively. This erosion and dilation was also used to smooth the mask's edges. In future iterations, the hole filling function imclose might be moved after this second debris clearing function, as imclose might interfere with imerode's ability to separate overlapping objects.

Masking

Finally, the binary mask was applied to the grayscale image in an element-wise matrix multiplication, returning a grayscale image of just the cell or nucleus in question.

Questions & Answers

what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
research.net
kanaga
sciencedirect big data base
Ernesto
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
Bharti
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
Daniel
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Automatic white blood cell classification using svm and neural networks. OpenStax CNX. Dec 16, 2015 Download for free at http://legacy.cnx.org/content/col11924/1.5
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