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Segmentation block diagram


Green channel isolation

As all images were in RGB format, green channel isolation simply involved taking the green layer of the matrix containing the image, returning a grayscale image based on the green intensity of the original image. Recall that this was done to match the white blood cell stains.


Thresholding was performed on the grayscale images with MATLAB's multithresh function using Otsu's method, separating the image with 5 different thresholds into 6 different levels. The 2nd most exclusive threshold was used to select nuclei, and the 4th most exclusive threshold was used to select cell bodies, returning two rough binary masks.


These rough masks were then processed to remove debris, fill holes, and smooth edges.
  • Debris was removed by deleting all objects with a centroid within a fixed distance from the margins or smaller than a fixed number of pixels. The objects and their centroids were found using MATLAB's regionprops function.
  • Holes were filled using MATLAB's imfill function and imclose function.
  • Since debris overlapping with the cell or nucleus of interest were not removed with the previous debris clearing function, a binary erosion was performed on the mask, the debris clearing function was repeated, and a binary dilation was performed on the mask, using MATLAB's imerode and imdilate functions, respectively. This erosion and dilation was also used to smooth the mask's edges. In future iterations, the hole filling function imclose might be moved after this second debris clearing function, as imclose might interfere with imerode's ability to separate overlapping objects.


Finally, the binary mask was applied to the grayscale image in an element-wise matrix multiplication, returning a grayscale image of just the cell or nucleus in question.

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
what school?
biomolecules are e building blocks of every organics and inorganic materials.
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
sciencedirect big data base
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Automatic white blood cell classification using svm and neural networks. OpenStax CNX. Dec 16, 2015 Download for free at http://legacy.cnx.org/content/col11924/1.5
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