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View the results for Sequence 1. The first column of the results table identifieswhether or not the match is of type "family" or of type "domain".The family and domain names appear at the top of each box in the second column of the resultspage, the same column that contains the diagrams which show the localization of thesection of sequence that has been identified with the referenced family or domain.

How many matches were of the type "family"?

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What are the names of the families identified with this sequence?

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List any domains that were identified within Sequence 1.

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View the results for Sequence 2.

How many families were returned as matches?

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What families were identified with this sequence?

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List any domains that were identified within Sequence 2.

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View the results for Sequence 3.

How many families were returned as matches?

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What families were identified with this sequence?

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List any domains that were identified within Sequence 3.

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Return to the ExPASy Proteomics Tools server . Now, scroll down to the section entitled "post-translational modification prediction".Use NetPhos (4) to predict possible sites for serine, threonine and tyrosine phosphorylation on the three sequences above (all 3 sequences can be enteredas one query). Accept the default values and select "submit". For help interpreting the results, view the NetPhos output format .

How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 1?

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How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 2?

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How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 3?

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Are there any serine, threonine and tyrosine in the sequence that were not listed as a potential phosphorylation site? If so, explain why some of the residues were not listed as predicted phosphorylation sites. (Those uncertain about the answer to this question should view the above link explaining the output.)

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Once a protein sequence has been determined through proteomics techniques, bioinformatics can be used to predict certaintypes of topology. Topology is the sequence of secondary structure elements within a protein. The most basic secondary structure elements within proteins are the alpha helix, the beta sheet and the random coil. However, somealgorithms will predict topological features that are closely related to in vivo localization, such as signal sequences and transmembrane helices.

At the ExPASy Proteomics Tools server , scroll down on the ExPASy tools webpage to the section entitled "topology prediction". This sectioncontains tools that predict localization and sorting signals, as well as transmembrane regions within proteins. PSORT (5) is a computer programfor the prediction of protein localization. It requires input of an amino acid sequence and its source organism; and it searches for known,organism-specific protein sorting signals. It returns a list of candidate localization sites, accompanied by a score indicating the probability theprotein encoded by the input sequence would be localized to that site. To explore the use of PSORT, click on the PSORT link on the ExPASy tool page.Choose the "PSORT II" for eukaryotic sequences, and select the PSORT II Prediction. Cut and paste the following sequence for diacylglycerol kinase from Rattus norvegicus into the query box and click "Submit".

Questions & Answers

what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
research.net
kanaga
sciencedirect big data base
Ernesto
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
Bharti
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
Daniel
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Bios 533 bioinformatics. OpenStax CNX. Sep 24, 2008 Download for free at http://cnx.org/content/col10152/1.16
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