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View the results for Sequence 1. The first column of the results table identifieswhether or not the match is of type "family" or of type "domain".The family and domain names appear at the top of each box in the second column of the resultspage, the same column that contains the diagrams which show the localization of thesection of sequence that has been identified with the referenced family or domain.

How many matches were of the type "family"?

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What are the names of the families identified with this sequence?

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List any domains that were identified within Sequence 1.

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View the results for Sequence 2.

How many families were returned as matches?

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What families were identified with this sequence?

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List any domains that were identified within Sequence 2.

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View the results for Sequence 3.

How many families were returned as matches?

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What families were identified with this sequence?

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List any domains that were identified within Sequence 3.

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Return to the ExPASy Proteomics Tools server . Now, scroll down to the section entitled "post-translational modification prediction".Use NetPhos (4) to predict possible sites for serine, threonine and tyrosine phosphorylation on the three sequences above (all 3 sequences can be enteredas one query). Accept the default values and select "submit". For help interpreting the results, view the NetPhos output format .

How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 1?

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How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 2?

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How many (a) serine, (b) threonine, and (c) tyrosine phosphorylation sites are predicted for Sequence 3?

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Are there any serine, threonine and tyrosine in the sequence that were not listed as a potential phosphorylation site? If so, explain why some of the residues were not listed as predicted phosphorylation sites. (Those uncertain about the answer to this question should view the above link explaining the output.)

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Once a protein sequence has been determined through proteomics techniques, bioinformatics can be used to predict certaintypes of topology. Topology is the sequence of secondary structure elements within a protein. The most basic secondary structure elements within proteins are the alpha helix, the beta sheet and the random coil. However, somealgorithms will predict topological features that are closely related to in vivo localization, such as signal sequences and transmembrane helices.

At the ExPASy Proteomics Tools server , scroll down on the ExPASy tools webpage to the section entitled "topology prediction". This sectioncontains tools that predict localization and sorting signals, as well as transmembrane regions within proteins. PSORT (5) is a computer programfor the prediction of protein localization. It requires input of an amino acid sequence and its source organism; and it searches for known,organism-specific protein sorting signals. It returns a list of candidate localization sites, accompanied by a score indicating the probability theprotein encoded by the input sequence would be localized to that site. To explore the use of PSORT, click on the PSORT link on the ExPASy tool page.Choose the "PSORT II" for eukaryotic sequences, and select the PSORT II Prediction. Cut and paste the following sequence for diacylglycerol kinase from Rattus norvegicus into the query box and click "Submit".

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
research.net
kanaga
sciencedirect big data base
Ernesto
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
Bharti
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
Daniel
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
Maciej
characteristics of micro business
Abigail
for teaching engĺish at school how nano technology help us
Anassong
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
NANO
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
s.
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
Tarell
what is the actual application of fullerenes nowadays?
Damian
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
Tarell
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
Virgil
is Bucky paper clear?
CYNTHIA
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
NANO
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Harper
Do you know which machine is used to that process?
s.
how to fabricate graphene ink ?
SUYASH Reply
for screen printed electrodes ?
SUYASH
What is lattice structure?
s. Reply
of graphene you mean?
Ebrahim
or in general
Ebrahim
in general
s.
Graphene has a hexagonal structure
tahir
On having this app for quite a bit time, Haven't realised there's a chat room in it.
Cied
what is biological synthesis of nanoparticles
Sanket Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Bios 533 bioinformatics. OpenStax CNX. Sep 24, 2008 Download for free at http://cnx.org/content/col10152/1.16
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