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This module describes the many proteomics tools available from the ExPASy website. Tools are introduced for protein identification and characterization from amino acid composition, fingerprint mass spectroscopy and other mass spectroscopy techniques. Also included in this module is an introduction to profile and pattern searches, tools for predictions of post-translational protein modifications, tools for protein topology prediction, primary structure analysis, secondary structure prediction and tertiary structure prediction and visualization.

A proteome is the collection of all the proteins within a given organism, in the same way a genome is the collection of all the genes within a givenorganism. A proteome has some characteristics that are quite different from a genome, however. A principal difference is the fact that while a particularorganism will have the same set of identical DNA in any undamaged, healthy cell throughout its lifetime, the organism's proteins will differ greatly from onetissue to another, and from one life stage to another. Furthermore, proteins commonly incur a variety of chemical modifications after they are made.These modifications are critical for proper protein functioning and/or regulation, and moreover, these modifications cannot be determined withcertainty by looking at the DNA sequence alone. In a contempary high-throughput proteomics laboratory, the number of proteins identified and analyzed in oneday can be on the order of hundreds.

The term“proteome”was originally coined by an Australian scientist, Mark Wilkins (1), to describe the "PROTEin complement of the genOME". The term "proteomics" is used relatively loosely to describe any and all of thecollection of high throughput techniques that have emerged to enable the scientist to analyze all the proteins expressed under a certain set ofconditions within an individual cell or organism. The ExPASy (Expert Protein Analysis System) website (2), Swiss Institute of Bioinformatics, offers the definition that"proteomics can be defined as the qualitative and quantitative comparison of proteomes under different conditions to further unravel biological processes."

Common techniques for identifying the proteins within a proteome are 2D-PAGE (polyacrylamide gel electrophoresis) gels, amino acid (AA) composition analysis,peptide mass fingerprinting and other mass spectroscopy applications. A good starting point for becoming acquainted with 2D gels is the 2D PAGE tutorial offered by the Institute of Biological Sciences, University of Wales at Aberystwyth. ExPASy offers a good synopsis of peptide mass fingerprinting and AA composition analysis techniques, for those who are unfamiliar with these methods.

At the ExPASy Proteomics Tools server , the first category of tools are for protein identification and characterization. Take a look at the tools listedin this section. These tools are designed to identify the proteins that make up the proteome of study, using the data received from gels, AA analysis andmass spectroscopy experiments.

Questions & Answers

how can chip be made from sand
Eke Reply
is this allso about nanoscale material
Almas
are nano particles real
Missy Reply
yeah
Joseph
Hello, if I study Physics teacher in bachelor, can I study Nanotechnology in master?
Lale Reply
no can't
Lohitha
where is the latest information on a no technology how can I find it
William
currently
William
where we get a research paper on Nano chemistry....?
Maira Reply
nanopartical of organic/inorganic / physical chemistry , pdf / thesis / review
Ali
what are the products of Nano chemistry?
Maira Reply
There are lots of products of nano chemistry... Like nano coatings.....carbon fiber.. And lots of others..
learn
Even nanotechnology is pretty much all about chemistry... Its the chemistry on quantum or atomic level
learn
Google
da
no nanotechnology is also a part of physics and maths it requires angle formulas and some pressure regarding concepts
Bhagvanji
hey
Giriraj
Preparation and Applications of Nanomaterial for Drug Delivery
Hafiz Reply
revolt
da
Application of nanotechnology in medicine
has a lot of application modern world
Kamaluddeen
yes
narayan
what is variations in raman spectra for nanomaterials
Jyoti Reply
ya I also want to know the raman spectra
Bhagvanji
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
Nasa has use it in the 60's, copper as water purification in the moon travel.
Alexandre
nanocopper obvius
Alexandre
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
if virus is killing to make ARTIFICIAL DNA OF GRAPHENE FOR KILLED THE VIRUS .THIS IS OUR ASSUMPTION
Anam
analytical skills graphene is prepared to kill any type viruses .
Anam
Any one who tell me about Preparation and application of Nanomaterial for drug Delivery
Hafiz
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Bios 533 bioinformatics. OpenStax CNX. Sep 24, 2008 Download for free at http://cnx.org/content/col10152/1.16
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