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As a demonstration, view the Stanford Microarray Database website. Under the Public Data section, click on the "Public Login" link. Limit the data set search to the organism Arabidopsis thaliana and the author Gutierrez. Click on the button entitled "Display data", and a table of microarray datasets should be returned. Choose one of the experiments, making note of the Experiment ID number from the table and select the clickable image icon. (There is a legend for the icons at the top of the web page, if there is uncertainty as to which is the clickable image icon.) This yields the qualitative visualization of the microarray experiment, as described previously in this module. Take a look at the array image and note that it is difficult to draw many conclusions fromthis kind of visualization.

What is the Experiment ID number for the viewed microarray image?

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Is it possible to get a feeling for which color dot, green, red, or yellow, is most predominant just by viewing the image? (If so, which color?)

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Are all of the dots, over the entire grid, well-shaped? (Give a brief explanation.)

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Click on one of the individual spots in the microarray grid. This will open a new window that contains a close up of the individual spot and all the experimental information about that spot.

Retrieve and list the following information about the chosen spot: a. spot number, b. description (under biological information), c. the Channel 1 intensity (mean), d. the Channel 1 background (median), e. the Channel 1 net intensity (mean), and f. the Log(base2) of R/G Normalized Ratio (Mean).

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Return to the table of Arabidopsis thaliana data sets and forExperiment ID #11374, select the "Data" icon, which is the first icon under the "Options" column. Next to "Sort By", select "Log(base2) of R/G NormalizedRatio (Mean)", and "Descending". Under "Display:", click on "Spot", then scroll down and hold down the control key (or the apple key on macintoshes) while selecting "Log(base2) ofR/G Normalized Ratio (Mean)". (The control key allows selection of additional choices without deselecting the previous choice.) Accept the default values for allremaining options and select "Display" at the bottom of the page. Recall that the data are converted to numbers representingthe fluorescence intensity of red dye, green dye, and the ratios of red to green. Scientists commonly use a log transformation of the ratio data, because thelogs are more mathematically tractable in reference to statistical analysis. The results page will show the top ranking spots from this chip, ranked from highest log red/green value to lowest.

What are the spot numbers of the three highest ranking spots?

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What are the "Log(base2) of R/G Normalized Ratio (Mean)" values for the three highest ranking spots?

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Use the browser's back button to go back and select new data for display. Chage the sortingselection to R/G Normalized (Mean), in descending order. under the "Display:" window select "Spot", then scroll down and hold down the control key while selecting "R/G Normalized (Mean)".

Questions & Answers

where we get a research paper on Nano chemistry....?
Maira Reply
what are the products of Nano chemistry?
Maira Reply
There are lots of products of nano chemistry... Like nano coatings.....carbon fiber.. And lots of others..
learn
Even nanotechnology is pretty much all about chemistry... Its the chemistry on quantum or atomic level
learn
Google
da
no nanotechnology is also a part of physics and maths it requires angle formulas and some pressure regarding concepts
Bhagvanji
Preparation and Applications of Nanomaterial for Drug Delivery
Hafiz Reply
revolt
da
Application of nanotechnology in medicine
what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
Nasa has use it in the 60's, copper as water purification in the moon travel.
Alexandre
nanocopper obvius
Alexandre
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
if virus is killing to make ARTIFICIAL DNA OF GRAPHENE FOR KILLED THE VIRUS .THIS IS OUR ASSUMPTION
Anam
analytical skills graphene is prepared to kill any type viruses .
Anam
Any one who tell me about Preparation and application of Nanomaterial for drug Delivery
Hafiz
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Bios 533 bioinformatics. OpenStax CNX. Sep 24, 2008 Download for free at http://cnx.org/content/col10152/1.16
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