<< Chapter < Page Chapter >> Page >

    Topics in this module

  • Applications of Molecular Distance Measures
    • Protein Classification
    • Protein Alignment
  • Local Matching: Geometric Hashing, Pose Clustering, and Match Augmentation

In a previous module , the topic of comparing and quantifying the distance between different conformations of a given molecule was explored. Structure-based comparison is also of interest for distinct proteins, which lack the atom-by-atom correspondence necessary for RMSD calculations. In this case, an alignment is performed either based on amino acid sequence or on three-dimensional structure, and the subset of atoms successfully aligned are used as the basis for calculating conformational distance. Computing distances among entire proteins by doing a global alignment of their structures is useful for protein classification.

Protein classification

Protein classification is motivated by the notion of "descriptive biology". When faced with tremendous amounts of highly complex data, such as with the set of all proteins, one way to understand the data is by classification: the act of associating or grouping proteins into classes using certain criteria. One such criterion is protein sequence identity, where sequential similarity led to the development of phylogenetic trees and multiple sequence analyses. The same is done in protein structure classification, where the effort is to identify groups of similar proteins, with the hope that this will yield information about their biochemical function and biological purpose.

Proteins are classified by simultaneously applying a number of criteria, including sequence homology (evolutionary relatedness), function, folding motifs, structural features, and so on. The resulting hierarchies and clusters of protein structures provide a notion of the distance between two proteins and their structures. A couple of popular classification schemes are linked below. Note that a fair amount of manual annotation and classification was necessary to build these systems.

Protein alignment

The core computational problem of protein classification, using sequence or structure, is the problem of comparing two proteins. For structural classification, one method for comparison is structural alignment , which identifies an ideal superimposition between two protein structures, in order to compare them.

SSAP, Dali, Foldminer, Lock, and Geometric Hashing are algorithms which have been designed in part to align whole protein structures. Despite differences in algorithmic approach, all of these algorithms essentially evolved from the need to assign the best possible correlation between points in one structure and points in another. The problem of finding the optimal alignment is polynomial in the number of atoms in biological data, where we are assured that atoms cannot fall within a certain distance to each other (Van der Waals forces enforce this), but without this constraint the problem is exponential.

Protein alignment has been used for the classification and comparison of proteins in many existing algorithms. These include:

  • Dali is a structural comparison algorithm based on pairwise distance matrices. Dali uses patterns of residue contacts, similar to contact maps described above in the intramolecular distances section, in order to align structures. The alignments are found using a randomized (Monte Carlo) search.
  • FoldMiner and LOCK 2 . FoldMiner finds protein structures similar to an input structure by performing alignment the query structures secondary structure elements with proteins in its database using the LOCK 2 algorithm. LOCK 2 uses a combination of geometric hashing and dynamic programming to optimize the alignments of secondary structure elements of different proteins. Once a set of alignments to similar structures are found, motifs consisting of similar secondary structure arrangements are constructed and used to refine the similarity search.
  • Sequential Structure Alignment Program (SSAP) Given two protein structures, SSAP returns a structural alignment.

Questions & Answers

what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
research.net
kanaga
sciencedirect big data base
Ernesto
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
Bharti
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
Daniel
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
Privacy Information Security Software Version 1.1a
Good
Got questions? Join the online conversation and get instant answers!
Jobilize.com Reply

Get the best Algebra and trigonometry course in your pocket!





Source:  OpenStax, Geometric methods in structural computational biology. OpenStax CNX. Jun 11, 2007 Download for free at http://cnx.org/content/col10344/1.6
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Geometric methods in structural computational biology' conversation and receive update notifications?

Ask