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To screen a genomic library for a particular gene or sequence of interest, researchers must know something about that gene. If researchers have a portion of the sequence of DNA for the gene of interest, they can design a DNA probe , a single-stranded DNA fragment that is complementary to part of the gene of interest and different from other DNA sequences in the sample. The DNA probe may be synthesized chemically by commercial laboratories, or it may be created by cloning, isolating, and denaturing a DNA fragment from a living organism. In either case, the DNA probe must be labeled with a molecular tag or beacon, such as a radioactive phosphorus atom (as is used for autoradiography ) or a fluorescent dye (as is used in fluorescent in situ hybridization, or FISH), so that the probe and the DNA it binds to can be seen ( [link] ). The DNA sample being probed must also be denatured to make it single-stranded so that the single-stranded DNA probe can anneal to the single-stranded DNA sample at locations where their sequences are complementary. While these techniques are valuable for diagnosis, their direct use on sputum and other bodily samples may be problematic due to the complex nature of these samples. DNA often must first be isolated from bodily samples through chemical extraction methods before a DNA probe can be used to identify pathogens.

A diagram of DNA probe. First a gene of interest is identified and cloned. Then single stranded probes are labeled with a molecular beacon. Finally, the DNA probe binds to complementary sequences in a DNA sample. The complementary sequences are single stranded DNA. The probe only attaches to one of the ssDNA sequences since it has the gene of interest in it
DNA probes can be used to confirm the presence of a suspected pathogen in patient samples. This diagram illustrates how a DNA probe can be used to search for a gene of interest associated with the suspected pathogen.

Part 2

The mild, flu-like symptoms that Kayla is experiencing could be caused by any number of infectious agents. In addition, several non-infectious autoimmune conditions, such as multiple sclerosis, systemic lupus erythematosus (SLE), and amyotrophic lateral sclerosis (ALS), also have symptoms that are consistent with Kayla’s early symptoms. However, over the course of several weeks, Kayla’s symptoms worsened. She began to experience joint pain in her knees, heart palpitations, and a strange limpness in her facial muscles. In addition, she suffered from a stiff neck and painful headaches. Reluctantly, she decided it was time to seek medical attention.

  • Do Kayla’s new symptoms provide any clues as to what type of infection or other medical condition she may have?
  • What tests or tools might a health-care provider use to pinpoint the pathogen causing Kayla’s symptoms?

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Agarose gel electrophoresis

There are a number of situations in which a researcher might want to physically separate a collection of DNA fragments of different sizes. A researcher may also digest a DNA sample with a restriction enzyme to form fragments. The resulting size and fragment distribution pattern can often yield useful information about the sequence of DNA bases that can be used, much like a bar-code scan, to identify the individual or species to which the DNA belongs.

Gel electrophoresis is a technique commonly used to separate biological molecules based on size and biochemical characteristics, such as charge and polarity. Agarose gel electrophoresis is widely used to separate DNA (or RNA) of varying sizes that may be generated by restriction enzyme digestion or by other means, such as the PCR ( [link] ).

Questions & Answers

fermented foods likely benefited ancestors to preserve foods and make other foods like milk, cheese, and bread using microbes.
Zinnia Reply
the evidence that I would support the statement regarding ancient people ideas that disease was transmitted by things they could not see is by the discoveries under a microscope. Many microbiologists have discovered certain diseases caused by microbes.
Zinnia
plz give the information about glyoxylate cycle
kamini Reply
what type of information?
Sapiens
I need medical microbiology mcqs books for Mbbs
Kisota
for pakistani mbbs or other?
Sapiens
what is microbial soup?
Osborn Reply
😃😂funny question isn't it?!!! sorry for inconvenience 😊
ayesha
most bacterial pathogens that cause disease in humans are classified in terms if their preferable ph as
Beesan Reply
what is micro
Kabul Reply
extremely small in size
Swetha
almost invisible to the naked eye
Peter
thanks
Sadiq
uwc
Peter
invisible sized objects
Sivasri
invisible sized object or specimens.which is used to see in only the microscope that specimens or objective called the micro
Sivasri
small organism that can be seen by use of Microbes
Manyang
why do you need to study microorganisms
Sala
what is microbiology?
Green Reply
microbiology is a branch of biology .it deals with the study of microorganisms life cycle , uses, disadvantages,and it impact in any other fields .which is used to view in microscope .
Sivasri
Microbiology is the study of microorganisms, those being unicellular, multicellular, or acellular. Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology and parasitology. 
Raj
In short , it is the study of micro organisms and types , characteristics.
Kaviya
can you explain the structure of rna in detail
Dhanalakshmi Reply
RNA is of many types....like mRNA,tRNA,rRNA,snRNA,guideRNA..etc .
Swetha
but generally RNA is single stranded
Swetha
It contains adenine guanine cytosine , instead of thymine it contains uracil.
Swetha
ribonucleic acid present in RNA.. where as in DNA it is deoxyribonucleic acid...and ribonucleotide is present in RNA
Swetha
RNA is a blue print of DNA. it has the information from DNA....and we can predict the base panirs in DNA if we have the RNA....copy of that DNA
Swetha
can either have positive or negative polarity
Ernestine
Diatoms need..... With the help of which they can construct their beautiful cell wall
Prathmesh Reply
Microbial growth curve shows a.... Curve.
Shambhuraj Reply
diminishing curve
Oluwapamilerin
It represents the decreasing growth of an organism.
Swetha
what is meant by reverse isolation in tuberculosis treatment?
Swetha Reply
why do we study microbiology
HABIBA Reply
Microbiology is the way of gaining knowledge about those organisms which are surround us but we can't see them with our naked eye,such organisms called microorganisms..We can see them only under microscope.
ayesha
from our birth to death microorganisms are with us in the environment as well as on our body which are called Microflora.
ayesha
Microfloras are non pathogenic
ayesha
what is microbiology
Anji Reply
It is the study of microorganisms and their structures functions and their interactions with environment ...
Swetha
it is the study of microorganisms which are invisible to backed eye but we can see them under microscope then microbiology is the study of microorganisms including their structures , functions,and their interactions with environment
Swetha
tq
Anji
tqq...not backed eye sorry nacked eye
Swetha
it's ok
Anji
where r u from
Anji
thank you
Swetha
from India
Swetha
which state
Anji
Hyderabad .
Swetha
so telugu vosthadhi kadha
Anji
chelusu nakku kochum
Swetha
thank you
Swetha
what is reverse isolation in tuberculosis treatment?
Swetha
Reverse isolation is used to protect you from germs when your immune system is not working properly. Germs can be carried on droplets in the air, medical equipment, or another person's body or clothing.
Kaviya
will you repeat this again?
Swetha
Reverse isolationis used to protect you from germs when your immune system is not working properly. Germs can be carried on droplets in the air, medical equipment, or another person's body or clothing.
Kaviya
kill you?
Swetha
Y
Kaviya
nothing to see the whole answer that is why I sent u like that then only I can see the full answer la
Swetha
What is allosteric site?
Hetshree Reply
I don't know how to explain but it's a deep concave site of an enzyme it's slightly or more slightly similar to the active site on an enzyme
ayesha
a site other than enzyme active site
Muhammad
describe the gram staining procedure as used in identification and classification of bacteria and it's interpretation
tabitha Reply
Gram staining method found by Hans Christian Gram. Classified into Gram Positive and Gram Negative Positive would be purple or blue stained bacteria Negative would be Pink or Red stained bacteria Both are observed under microscope This procedure performed on glass slides by making a smear of sample.
ayesha
Thanks.. nicely u defined
shamim
jazakallah Khair
ayesha
hmmm
shamim
Masha allah
Khamis
jazakallah Khair
ayesha
hi
Nafiu
Heavy rains on Tuesday 21/1/20202 caused heavy floods on wednesday of 22/1/2020. Floods caused a lot of destruction including cut off of central corridor main road, property destruction and other problems including a single reported death. As a clinician you are asked to mention and explain 5 common health problems in all disasters.
Miryam Reply

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Source:  OpenStax, Microbiology. OpenStax CNX. Nov 01, 2016 Download for free at http://cnx.org/content/col12087/1.4
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