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Alcohols tend to be bactericidal and fungicidal, but may also be viricidal for enveloped viruses only. Although alcohols are not sporicidal, they do inhibit the processes of sporulation and germination. Alcohols are volatile and dry quickly, but they may also cause skin irritation because they dehydrate the skin at the site of application. One common clinical use of alcohols is swabbing the skin for degerming before needle injection. Alcohols also are the active ingredients in instant hand sanitizer s, which have gained popularity in recent years. The alcohol in these hand sanitizers works both by denaturing proteins and by disrupting the microbial cell membrane, but will not work effectively in the presence of visible dirt.

Last, alcohols are used to make tincture s with other antiseptics, such as the iodine tinctures discussed previously in this chapter. All in all, alcohols are inexpensive and quite effective for the disinfection of a broad range of vegetative microbes. However, one disadvantage of alcohols is their high volatility, limiting their effectiveness to immediately after application.

a) Ethyl alcohol has 2 C’s and an OH.  B) Isopropyl alcohol has 3 C’s and an OH.
(a) Ethyl alcohol, the intoxicating ingredient found in alcoholic drinks, is also used commonly as a disinfectant. (b) Isopropyl alcohol, also called rubbing alcohol, has a related molecular structure and is another commonly used disinfectant. (credit a photo: modification of work by D Coetzee; credit b photo: modification of work by Craig Spurrier)
  • Name at least three advantages of alcohols as disinfectants.
  • Describe several specific applications of alcohols used in disinfectant products.

Surfactants

Surface-active agents, or surfactants , are a group of chemical compounds that lower the surface tension of water. Surfactants are the major ingredients in soaps and detergents . Soaps are salts of long-chain fatty acids and have both polar and nonpolar regions, allowing them to interact with polar and nonpolar regions in other molecules ( [link] ). They can interact with nonpolar oils and grease to create emulsions in water, loosening and lifting away dirt and microbes from surfaces and skin. Soaps do not kill or inhibit microbial growth and so are not considered antiseptics or disinfectants. However, proper use of soaps mechanically carries away microorganisms, effectively degerming a surface. Some soaps contain added bacteriostatic agents such as triclocarban or cloflucarban , compounds structurally related to triclosan, that introduce antiseptic or disinfectant properties to the soaps.

A chemical structure with a long carbon chain and two oxygens at one end. The end with the oxygens is the hydrophobic head which shuns hydrocarbon-like substances but is attracted to water molecules. Thi sis the anionic portion of the molecule. The long carbon chains are the hydrophilic tail which shuns water but is attracted to oily greasy hydrocarbon-like substances.
Soaps are the salts (sodium salt in the illustration) of fatty acids and have the ability to emulsify lipids, fats, and oils by interacting with water through their hydrophilic heads and with the lipid at their hydrophobic tails.

Soaps, however, often form films that are difficult to rinse away, especially in hard water, which contains high concentrations of calcium and magnesium mineral salts. Detergents contain synthetic surfactant molecules with both polar and nonpolar regions that have strong cleansing activity but are more soluble, even in hard water, and, therefore, leave behind no soapy deposits. Anionic detergents , such as those used for laundry, have a negatively charged anion at one end attached to a long hydrophobic chain, whereas cationic detergents have a positively charged cation instead. Cationic detergents include an important class of disinfectants and antiseptics called the quaternary ammonium salts (quats) , named for the characteristic quaternary nitrogen atom that confers the positive charge ( [link] ). Overall, quats have properties similar to phospholipids, having hydrophilic and hydrophobic ends. As such, quats have the ability to insert into the bacterial phospholipid bilayer and disrupt membrane integrity. The cationic charge of quats appears to confer their antimicrobial properties, which are diminished when neutralized. Quats have several useful properties. They are stable, nontoxic, inexpensive, colorless, odorless, and tasteless. They tend to be bactericidal by disrupting membranes. They are also active against fungi, protozoans, and enveloped viruses, but endospores are unaffected. In clinical settings, they may be used as antiseptics or to disinfect surfaces. Mixtures of quats are also commonly found in household cleaners and disinfectants, including many current formulations of Lysol brand products, which contain benzalkonium chlorides as the active ingredients. Benzalkonium chlorides, along with the quat cetylpyrimidine chloride , are also found in products such as skin antiseptics, oral rinses, and mouthwashes.

Questions & Answers

is it better to study microbiology and then medicine it makes no difference to go directly to medicine?
Jessee Reply
Dray's mathdme cell wall konse color k hote he
Jinal Reply
what is dray's mathdme cell wall
Prabhat
I confused. please help me
Karen
just confused
Raj
l don't understand it please explain it for me.
Karen Reply
epitopes are present on the surface of
Rohit Reply
at the tip of variable region on the antibody...where antigen and antibody binding sites combine...
Fiza
The term that is used refer to moving microbes under a microscope are referred to as?
Lee Reply
Members of the genus Neisseria cause which of the folowing human diseases?
Farah Reply
genital infections
Kamaluddeen
gonorrhoea
sandip
gonorrhoea
Jessee
4. Which of the following specimens should not be refrigerated? a. Urine b. Urogenital swab
Zahraa Reply
urine
Muuse
urine
Agatha
Urine
Tean
urine
Yasser
Urine
Ebtehal
urine
anamika
urine
Puja
urine
Inemesit
urine
Samuel
Urine
Muhammad
Details about McConkey agar
Muhammad
urine
SK
urine
what is bacteria
anamika Reply
a member of large number of unicellular microorganism which have cell wall but lack of cell organelles an oranised nucleus including somewhat can cause disease
Sukhdeep
Bacteria are usually composed of one cell onl to that are neither plants nor animals, microscopic, that may cause diseases or may be beneficial(in gut)... it depends upon their weapons. Nearly all animal life is dependent on Bacteria for their survival
Fiza
thanks
anamika
what factor make bacteria colony large and how could we sterlise it in large scale
fatty
nutrient concentration temp gaseous conc ph ion or salt concentration mositure condition factors contribute to make large colony. by autoclaving we will sterilize bactetia
Sukhdeep
Colony is actually visible growth of Bacteria that is as a result of suitable environment for growth i.e optimal conditions for growth, temperature, moisture etc. there're many methods to get rid of bacteria. If We stop giving them optimal conditions for living Bacteria will die soon .
Fiza
what's the difference between an antigen and a pathogen?
Pathogens are organisms that cause disease in other organisms whereas Antigen is a part of a pathogen that triggers the immune response..
Rajat
so it is the antigen that dendritic cells present to the T cells and not the pathogen itself?
no no antigen are the west product or part of the pathogen. in such case bacteria it self fight with over immune response & in another case bacteria release antigens
vasava
& other antigen like pollan grain, dust particles etc.....
vasava
pathogen are microbes that can infect the body and causw illness....antigens are the part of pathogens that alert the body to an infection
Sukhdeep
antigen is a part of blood and pathogen is foreign particle which causes diseases
Yogyata
antigen could be non microorganism.... where as pathogen is mixroorganism
tadesse
Thanks
Karen
a pathogen is a disease causing organism while an antigen is a protein in the white blood cells which combats pathogens.
Jessee
what type of widal test
sobhit Reply
this test determine for typhoid in this test if H,O antigen are present that indicate the positive test bac. are salmonella typhy
vasava
what h.o denotes
Iqra
o: body of bacteria, h: flagellate
Explain Mould
Chinenye Reply
Explain mycoses and it's classification
Chinenye
why do we have hiccups?
Manisha Reply
shakey diaphragm
Curlisse
The antibody binding site is formed primarily by:
Asalla Reply
How many types of MICROORGANISMS do we have?
Hope Reply
Hello friends
Hope
microorganisms are divided into seven type Bacteria archaea protozoa algae fungi virus and multicellular animal parasites
Raj
What's pathogenesity
Usman Reply
something that causes disease
Anietie
who is the father of microbiology?
Hope
antonie van leeuwenhoek
vasava
it is the severity of the effects caused by a pathogen
Jessee

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Source:  OpenStax, Microbiology. OpenStax CNX. Nov 01, 2016 Download for free at http://cnx.org/content/col12087/1.4
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