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Primary structure

The unique sequence of amino acids in a polypeptide chain is its primary structure    . For example, the pancreatic hormone insulin has two polypeptide chains, A and B, and they are linked together by disulfide bonds. The N terminal amino acid of the A chain is glycine, whereas the C terminal amino acid is asparagine ( [link] ). The sequences of amino acids in the A and B chains are unique to insulin.

The amino acid sequences for the A chain and B chain of bovine insulin are shown. The A chain is 21 amino acids in length, and the B chain is 30 amino acids in length. One disulfide, or SS bond, connects two cysteine residues in the A chain. Two other disulfide linkages connect the A chain to the B chain.
Bovine serum insulin is a protein hormone made of two peptide chains, A (21 amino acids long) and B (30 amino acids long). In each chain, primary structure is indicated by three-letter abbreviations that represent the names of the amino acids in the order they are present. The amino acid cysteine (cys) has a sulfhydryl (SH) group as a side chain. Two sulfhydryl groups can react in the presence of oxygen to form a disulfide (S-S) bond. Two disulfide bonds connect the A and B chains together, and a third helps the A chain fold into the correct shape. Note that all disulfide bonds are the same length, but are drawn different sizes for clarity.

The unique sequence for every protein is ultimately determined by the gene encoding the protein. A change in nucleotide sequence of the gene’s coding region may lead to a different amino acid being added to the growing polypeptide chain, causing a change in protein structure and function. In sickle cell anemia, the hemoglobin β chain (a small portion of which is shown in [link] ) has a single amino acid substitution, causing a change in protein structure and function. Specifically, the amino acid glutamic acid is substituted by valine in the β chain. What is most remarkable to consider is that a hemoglobin molecule is made up of two alpha chains and two beta chains that each consist of about 150 amino acids. The molecule, therefore, has about 600 amino acids. The structural difference between a normal hemoglobin molecule and a sickle cell molecule—which dramatically decreases life expectancy—is a single amino acid of the 600. What is even more remarkable is that those 600 amino acids are encoded by three nucleotides each, and the mutation is caused by a single base change (point mutation), 1 in 1800 bases.

A portion of the hemoglobin amino acid sequence is shown. The normal hemoglobin beta chain has a glutamate at position six. The sickle cell beta chain has a valine at this position.
The beta chain of hemoglobin is 147 residues in length, yet a single amino acid substitution leads to sickle cell anemia. In normal hemoglobin, the amino acid at position seven is glutamate. In sickle cell hemoglobin, this glutamate is replaced by a valine.

Because of this change of one amino acid in the chain, hemoglobin molecules form long fibers that distort the biconcave, or disc-shaped, red blood cells and assume a crescent or “sickle” shape, which clogs arteries ( [link] ). This can lead to myriad serious health problems such as breathlessness, dizziness, headaches, and abdominal pain for those affected by this disease.

This electron micrograph shows red blood cells from a patient with sickle cell anemia. Most of the cells have a normal, disk shape, but about one in five has a sickle shape. A normal blood cell is eight microns across.
In this blood smear, visualized at 535x magnification using bright field microscopy, sickle cells are crescent shaped, while normal cells are disc-shaped. (credit: modification of work by Ed Uthman; scale-bar data from Matt Russell)

Questions & Answers

simple definition for respiratory system
Gift Reply
Respiratory system is a network of organs and tissues that helps you to breathe or help in getting the rids of oxygen and discharge of carbon dioxide in the body.
Ruhiyatu
how is a aerenchyma tissue adapted to its function
fatuma Reply
what does DNA mean
innocent Reply
dioxiribo nucleic acid
Lekan
what is a zygote
Victor Reply
zygote is developed or fertilized egg cell
Lekan
what is the difference between cell wall and cell membrane
Ruhiyatu Reply
cell wall is found in plant while cell membrane is found in animal cell
Lekan
please this is the main answer to that question okay Cell wall gives shape and support to the cell whiles Cell membrane support the movement of substances into and out of the cell. This question is very tricky that's why I asked.
Ruhiyatu
how cell I form
Elijah Reply
what difference between animal cell and plant cell
Lazarus Reply
what is animal call
Rita
Animal cell does not have a cell wall but plant cell have a cell wall
Ruhiyatu
plant have cell Wall well animal have not cell wall
Yula
what is fertilization?
Muhamed Reply
Fertilization is the fusion of male sex cell and female sex cell to form a zygote.
Ruhiyatu
What kind of nutrients is composed of plants
Annie Reply
phosphorus,potassium,nitrogen
Kritika
NPK
Ruhiyatu
what is hormones
Igwe Reply
hormon is the chemical messanger
Sneha
Genes can make someone dull?
Taperah
21ecological instrument and their diagrams
Ayomide Reply
cell biology
Muhamed
I am sorry
onuoha
for wat
ezeadaugo
no reason
KP
nothing but speculate
KP
Sorry without reason is nothing but speculate
Leilah
what are the kidney disease
Immaculate Reply
kidney stones
Gracelyne
it is a disease that affects the kidney
Miriam
what are some lung diseases
Gracelyne
kidney cancer
Victor
what is the other way of treating kidney failure
Mirriam
what is micro-organism
Jackson Reply
what is the hypothesis
Jackson
hypothesis is a proposed explanation for a phenomenon
Miriam
hypothesis is raw materials
KP
what is biology
KP
biology is the study of living things and their interaction with their environment
Miriam
what is zyogot?
Muhamed
what is fertilization?
Muhamed
zygote is an unfertilized eggs
KP
fertilization refers to the fusion of a sperm and ovum
KP
organs that can't be seen physically
Victor
what does mean stigma
Amira Reply
the stigma receives pollen grains during pollination
Ruhiyatu
what is the full of the MOST dangerous disease in the world where one stops sleeping and just dies :Hint ; FFI
God Reply
fatal familial insomnia which affects the thalamus
Miriam
there are other dangerous diseases like CAD i.e coronary artery disease
Miriam

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Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
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