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Image acquisition

The optical-fiber system

We have designed an optical system to test the imaging capabilities of a one millimeter diameter optical fiber probe. We used a 455 nm Luxeon LED (Philips, San Jose, CA) as the illumination source in accordance with our design goal of a device capable of both reflectance and fluorescence imaging while remaining as simple as possible. A broadband light source would be more ideal for reflectance imaging. However, a broadband light source could perform fluorescence imaging whereas our source would be suitable for both fluorescence and reflectance imaging. The LED was cooled by a heat sink and fan assembly mounted on its back and powered by a 12V AC/DC adaptor. To mimic the fatty environment of the human breast, test images were taken from samples of chicken adipose tissue.

The 1-mm diameter fiber (Sumitomo Electric USA, Los Angeles, CA) was positioned next to the LED and over a vertically adjustable sample stage. (Figure 2) The fiber tip was adjusted to receive maximum illumination from the LED when it was in contact with the sample. The fiber collected the light reflected by the sample at its distal end and conveyed it to its proximal end. The proximal end of the fiber was focused at the focal plane of an infinity corrected microscope objective (Newport, Irvine, CA). The position of the fiber was adjusted by a three-axis micro translation stage (Newport). The infinity corrected object could project an image from its rear that is in focus regardless of the distance between the object and the detector.

The detector was a Zeiss MRc5 5.0 megapixels color CCD camera (Carl Zeiss, Thornwood, NY). The magnification of the image on the detector relative to the image on the proximal fiber tip was the ratio between the distance from the detector to the back of the objective and the distance from the front of the objective to the fiber proximal tip. The infinity corrected objective permitted a wide range of magnifications (a while range of allowable detector-objective separation) while maintaining the focus of the image. (Figure 3) This feature could potentially allow the incorporation of a movable detector that can change the magnification of the image on command.

The image from the detector was captured onto a laptop computer (Lenovo, China) with the Zeiss AxioVision camera software. (Figure 4) The image was exported as a bitmap file. The bitmap file was subsequently processed in Matlab (Mathworks, Natick, MA).

Questions & Answers

where we get a research paper on Nano chemistry....?
Maira Reply
nanopartical of organic/inorganic / physical chemistry , pdf / thesis / review
Ali
what are the products of Nano chemistry?
Maira Reply
There are lots of products of nano chemistry... Like nano coatings.....carbon fiber.. And lots of others..
learn
Even nanotechnology is pretty much all about chemistry... Its the chemistry on quantum or atomic level
learn
Google
da
no nanotechnology is also a part of physics and maths it requires angle formulas and some pressure regarding concepts
Bhagvanji
hey
Giriraj
Preparation and Applications of Nanomaterial for Drug Delivery
Hafiz Reply
revolt
da
Application of nanotechnology in medicine
what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
Nasa has use it in the 60's, copper as water purification in the moon travel.
Alexandre
nanocopper obvius
Alexandre
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
if virus is killing to make ARTIFICIAL DNA OF GRAPHENE FOR KILLED THE VIRUS .THIS IS OUR ASSUMPTION
Anam
analytical skills graphene is prepared to kill any type viruses .
Anam
Any one who tell me about Preparation and application of Nanomaterial for drug Delivery
Hafiz
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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