<< Chapter < Page Chapter >> Page >

Image acquisition

The optical-fiber system

We have designed an optical system to test the imaging capabilities of a one millimeter diameter optical fiber probe. We used a 455 nm Luxeon LED (Philips, San Jose, CA) as the illumination source in accordance with our design goal of a device capable of both reflectance and fluorescence imaging while remaining as simple as possible. A broadband light source would be more ideal for reflectance imaging. However, a broadband light source could perform fluorescence imaging whereas our source would be suitable for both fluorescence and reflectance imaging. The LED was cooled by a heat sink and fan assembly mounted on its back and powered by a 12V AC/DC adaptor. To mimic the fatty environment of the human breast, test images were taken from samples of chicken adipose tissue.

The 1-mm diameter fiber (Sumitomo Electric USA, Los Angeles, CA) was positioned next to the LED and over a vertically adjustable sample stage. (Figure 2) The fiber tip was adjusted to receive maximum illumination from the LED when it was in contact with the sample. The fiber collected the light reflected by the sample at its distal end and conveyed it to its proximal end. The proximal end of the fiber was focused at the focal plane of an infinity corrected microscope objective (Newport, Irvine, CA). The position of the fiber was adjusted by a three-axis micro translation stage (Newport). The infinity corrected object could project an image from its rear that is in focus regardless of the distance between the object and the detector.

The detector was a Zeiss MRc5 5.0 megapixels color CCD camera (Carl Zeiss, Thornwood, NY). The magnification of the image on the detector relative to the image on the proximal fiber tip was the ratio between the distance from the detector to the back of the objective and the distance from the front of the objective to the fiber proximal tip. The infinity corrected objective permitted a wide range of magnifications (a while range of allowable detector-objective separation) while maintaining the focus of the image. (Figure 3) This feature could potentially allow the incorporation of a movable detector that can change the magnification of the image on command.

The image from the detector was captured onto a laptop computer (Lenovo, China) with the Zeiss AxioVision camera software. (Figure 4) The image was exported as a bitmap file. The bitmap file was subsequently processed in Matlab (Mathworks, Natick, MA).

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
what school?
biomolecules are e building blocks of every organics and inorganic materials.
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
sciencedirect big data base
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
Privacy Information Security Software Version 1.1a
Got questions? Join the online conversation and get instant answers!
Jobilize.com Reply

Get the best Algebra and trigonometry course in your pocket!

Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Detection of cell boundaries in optical fiber probe images' conversation and receive update notifications?