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Image acquisition

The optical-fiber system

We have designed an optical system to test the imaging capabilities of a one millimeter diameter optical fiber probe. We used a 455 nm Luxeon LED (Philips, San Jose, CA) as the illumination source in accordance with our design goal of a device capable of both reflectance and fluorescence imaging while remaining as simple as possible. A broadband light source would be more ideal for reflectance imaging. However, a broadband light source could perform fluorescence imaging whereas our source would be suitable for both fluorescence and reflectance imaging. The LED was cooled by a heat sink and fan assembly mounted on its back and powered by a 12V AC/DC adaptor. To mimic the fatty environment of the human breast, test images were taken from samples of chicken adipose tissue.

The 1-mm diameter fiber (Sumitomo Electric USA, Los Angeles, CA) was positioned next to the LED and over a vertically adjustable sample stage. (Figure 2) The fiber tip was adjusted to receive maximum illumination from the LED when it was in contact with the sample. The fiber collected the light reflected by the sample at its distal end and conveyed it to its proximal end. The proximal end of the fiber was focused at the focal plane of an infinity corrected microscope objective (Newport, Irvine, CA). The position of the fiber was adjusted by a three-axis micro translation stage (Newport). The infinity corrected object could project an image from its rear that is in focus regardless of the distance between the object and the detector.

The detector was a Zeiss MRc5 5.0 megapixels color CCD camera (Carl Zeiss, Thornwood, NY). The magnification of the image on the detector relative to the image on the proximal fiber tip was the ratio between the distance from the detector to the back of the objective and the distance from the front of the objective to the fiber proximal tip. The infinity corrected objective permitted a wide range of magnifications (a while range of allowable detector-objective separation) while maintaining the focus of the image. (Figure 3) This feature could potentially allow the incorporation of a movable detector that can change the magnification of the image on command.

The image from the detector was captured onto a laptop computer (Lenovo, China) with the Zeiss AxioVision camera software. (Figure 4) The image was exported as a bitmap file. The bitmap file was subsequently processed in Matlab (Mathworks, Natick, MA).

Questions & Answers

what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
research.net
kanaga
sciencedirect big data base
Ernesto
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
Bharti
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
Daniel
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
Maciej
characteristics of micro business
Abigail
for teaching engĺish at school how nano technology help us
Anassong
How can I make nanorobot?
Lily
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
NANO
how can I make nanorobot?
Lily
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
s.
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
Tarell
what is the actual application of fullerenes nowadays?
Damian
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
Tarell
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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