# 0.4 Connecting lines

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## Rationale

OK, so now we have a (relatively) clean image of the cell’s borders. Can we run an edge detector now you ask…? Hold your horses, Champ, we still have a ways to go…

Although it may make sense to run an edge detector at this point, seeing as we’ve got ourselves an image where the cell borders are definitely distinguishable, there is still a problem. Edge detectors look for changes in the gradient. Since our image was acquired utilizing optical fibers, our cell borders are not really solid lines quite yet; they are merely groups of small dots which, together, make up the cell borders. If an edge detector were utilized at this point, it would pick up each fiber optic probe, rather than the cell border we want. There no need to despair; there is a solution for all this!

## Mode filtering

Although it may not be as simple as those connect-the-dots books we’re used to, mode filtering can be an effective method of forming solid cell borders. The basic idea is this: if we used sliding neighborhood operations again, but rather than look for the mean, look for the mode, we may be able to connect all our little dots together! Why is this? Well, the mode filter we implemented works like this:

• Take a neighborhood of size [N M]
• Find the mean of the entries in the matrix.
• Since the image is in black and white, if the mean is greater than .5, then there are more ones, otherwise, there are more zeros. This is basically determining the mode of the neighborhood.
• Thus, if the mode is 1, set the pixel to 1. If the mode is 0, set the pixel to 0.

How does this connect the lines? Think about this: we want to establish solid cell borders by turning ON the black pixels which are part of the cell’s border. Thus, we want to turn ON black pixels which are near groups of white pixels which make up the cell borders and keep black pixels which are not part of the cell borders (in areas with relatively few white pixels) OFF. Taking the mode of each pixel’s neighborhood will accomplish this since black pixels near large groups of white pixels will be turned on and the following image shows what begins to look like solid cell borders… (Figure 1)

where we get a research paper on Nano chemistry....?
nanopartical of organic/inorganic / physical chemistry , pdf / thesis / review
Ali
what are the products of Nano chemistry?
There are lots of products of nano chemistry... Like nano coatings.....carbon fiber.. And lots of others..
learn
Even nanotechnology is pretty much all about chemistry... Its the chemistry on quantum or atomic level
learn
da
no nanotechnology is also a part of physics and maths it requires angle formulas and some pressure regarding concepts
Bhagvanji
hey
Giriraj
Preparation and Applications of Nanomaterial for Drug Delivery
revolt
da
Application of nanotechnology in medicine
what is variations in raman spectra for nanomaterials
I only see partial conversation and what's the question here!
what about nanotechnology for water purification
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
Nasa has use it in the 60's, copper as water purification in the moon travel.
Alexandre
nanocopper obvius
Alexandre
what is the stm
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
what is a peer
What is meant by 'nano scale'?
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
if virus is killing to make ARTIFICIAL DNA OF GRAPHENE FOR KILLED THE VIRUS .THIS IS OUR ASSUMPTION
Anam
analytical skills graphene is prepared to kill any type viruses .
Anam
Any one who tell me about Preparation and application of Nanomaterial for drug Delivery
Hafiz
what is Nano technology ?
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
why we need to study biomolecules, molecular biology in nanotechnology?
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
why?
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
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