<< Chapter < Page Chapter >> Page >

Control of catabolic pathways

Enzymes, proteins, electron carriers, and pumps that play roles in glycolysis, the citric acid cycle, and the electron transport chain tend to catalyze non-reversible reactions. In other words, if the initial reaction takes place, the pathway is committed to proceeding with the remaining reactions. Whether a particular enzyme activity is released depends upon the energy needs of the cell (as reflected by the levels of ATP, ADP, and AMP).

Glycolysis

The control of glycolysis begins with the first enzyme in the pathway, hexokinase ( [link] ). This enzyme catalyzes the phosphorylation of glucose, which helps to prepare the compound for cleavage in a later step. The presence of the negatively charged phosphate in the molecule also prevents the sugar from leaving the cell. When hexokinase is inhibited, glucose diffuses out of the cell and does not become a substrate for the respiration pathways in that tissue. The product of the hexokinase reaction is glucose-6-phosphate, which accumulates when a later enzyme, phosphofructokinase, is inhibited.

This illustration shows that glycolysis is regulated via three key enzymes: hexokinase phosphofructokinase, and phosphoglycerate kinase. The first two enzymes hydrolyze an ATP and the third one produces ATP.
The glycolysis pathway is primarily regulated at the three key enzymatic steps (1, 2, and 7) as indicated. Note that the first two steps that are regulated occur early in the pathway and involve hydrolysis of ATP.

Phosphofructokinase is the main enzyme controlled in glycolysis. High levels of ATP, citrate, or a lower, more acidic pH decrease the enzyme’s activity. An increase in citrate concentration can occur because of a blockage in the citric acid cycle. Fermentation, with its production of organic acids like lactic acid, frequently accounts for the increased acidity in a cell; however, the products of fermentation do not typically accumulate in cells.

The last step in glycolysis is catalyzed by pyruvate kinase. The pyruvate produced can proceed to be catabolized or converted into the amino acid alanine. If no more energy is needed and alanine is in adequate supply, the enzyme is inhibited. The enzyme’s activity is increased when fructose-1,6-bisphosphate levels increase. (Recall that fructose-1,6-bisphosphate is an intermediate in the first half of glycolysis.) The regulation of pyruvate kinase involves phosphorylation by a kinase (pyruvate kinase kinase), resulting in a less-active enzyme. Dephosphorylation by a phosphatase reactivates it. Pyruvate kinase is also regulated by ATP (a negative allosteric effect).

If more energy is needed, more pyruvate will be converted into acetyl CoA through the action of pyruvate dehydrogenase. If either acetyl groups or NADH accumulate, there is less need for the reaction and the rate decreases. Pyruvate dehydrogenase is also regulated by phosphorylation: A kinase phosphorylates it to form an inactive enzyme, and a phosphatase reactivates it. The kinase and the phosphatase are also regulated.

Citric acid cycle

The citric acid cycle is controlled through the enzymes that catalyze the reactions that make the first two molecules of NADH ( [link] ). These enzymes are isocitrate dehydrogenase and α -ketoglutarate dehydrogenase. When adequate ATP and NADH levels are available, the rates of these reactions decrease. When more ATP is needed, as reflected in rising ADP levels, the rate increases. α -Ketoglutarate dehydrogenase will also be affected by the levels of succinyl CoA—a subsequent intermediate in the cycle—causing a decrease in activity. A decrease in the rate of operation of the pathway at this point is not necessarily negative, as the increased levels of the α -ketoglutarate not used by the citric acid cycle can be used by the cell for amino acid (glutamate) synthesis.

Electron transport chain

Specific enzymes of the electron transport chain are unaffected by feedback inhibition, but the rate of electron transport through the pathway is affected by the levels of ADP and ATP. Greater ATP consumption by a cell is indicated by a buildup of ADP. As ATP usage decreases, the concentration of ADP decreases, and now, ATP begins to build up in the cell. This change is the relative concentration of ADP to ATP triggers the cell to slow down the electron transport chain.

Visit this site to see an animation of the electron transport chain and ATP synthesis.

For a summary of feedback controls in cellular respiration, see [link] .

Summary of Feedback Controls in Cellular Respiration
Pathway Enzyme affected Elevated levels of effector Effect on pathway activity
glycolysis hexokinase glucose-6-phosphate decrease
phosphofructokinase low-energy charge (ATP, AMP), fructose-6-phosphate via fructose-2,6-bisphosphate increase
high-energy charge (ATP, AMP), citrate, acidic pH decrease
pyruvate kinase fructose-1,6-bisphosphate increase
high-energy charge (ATP, AMP), alanine decrease
pyruvate to acetyl CoA conversion pyruvate dehydrogenase ADP, pyruvate increase
acetyl CoA, ATP, NADH decrease
citric acid cycle isocitrate dehydrogenase ADP increase
ATP, NADH decrease
α -ketoglutarate dehydrogenase Calcium ions, ADP increase
ATP, NADH, succinyl CoA decrease
electron transport chain ADP increase
ATP decrease

Section summary

Cellular respiration is controlled by a variety of means. The entry of glucose into a cell is controlled by the transport proteins that aid glucose passage through the cell membrane. Most of the control of the respiration processes is accomplished through the control of specific enzymes in the pathways. This is a type of negative feedback, turning the enzymes off. The enzymes respond most often to the levels of the available nucleosides ATP, ADP, AMP, NAD + , and FAD. Other intermediates of the pathway also affect certain enzymes in the systems.

Questions & Answers

mandle experiment point
Sarpz Reply
what is the mean of biology
chiluba
In a lay man definition biology talks about life as a whole
VICTOR
the study of living organisms
the study of the structure, function, and origin of living organisms
biology has many branches. Botany (the study of plants) , evolution,. Ecology (how animals interact amongst each other. the study the habitat of an animal and try to determine as well as find strategies to prevent a species from going extinct.
is simply the study of life
Namuli
hello
Namuli
what is a specicide
Twinkl Reply
why are mosquitoes primary and secondary consumers
Twinkl
what are the structures found in all viruses
Twinkl
how does vaccinating a woman prevent her baby from getting the illness?
Twinkl
how does selective breeding increase crop size
Twinkl
wat is an animal cell
Monicah Reply
what is deer
Khansawu Reply
breathing in reptiles
sam Reply
which molecules binds to the active site of an enzyme
Ghulam Reply
human
Haggai
please remind me
Bafaa
please I do not no
Bafaa
please remind me
Bafaa
please I do not no
Bafaa
please remind me
Bafaa
what is nutrients
Patrick Reply
nutrients is substance which body use in terms of need
Haggai
yes..
Abdullahi
can I join?
Osei
substance that provides nourishment essential for the maintenance of life and for growth.
umar
right same answer as you Umar
Namuli
please remind me
Namuli
Any material that provide essential components for cell metabolisms is nutrient
Mahmood
what are the types of skeleton
Tanyi Reply
endo skeleton and exo skeleton
Faith
or types of skeletons There is exo skeleton found in class insecta Exo skeleton found in phylum chordata and lastly hydrostatic skeleton found mainly in earth worms
Daniel
and hydrostatic skeleton
Godbless
bronah
Kapoko
endo, exo and hydrostatic skeleton
Mico
what is life?
Jerson Reply
life is when any living thing breath or respirate.
Lucky
I want to join
Patrick
me too
Favour
me two
Monday
me too
Bengazy
me too
Maggie
I here
Yahaya
Does anybody knows another biology app that brings good understand
Lucky
give an example of a phylum where all members have open circulatory
Omoro Reply
anthropoda
Daniel
phylum Anthropoda
Daniel
how do u change the unit of temperature from degree celsius to degree Fahrenheit
Maggie
what are beneficial effects in industrial
Nnamani Reply
create employment
ibrahim
provide food
ibrahim
pollution
ibrahim
dirty the environment
ibrahim
pollution and lack of knowledge about how to manage waste
Alex
what is gametophyte
Ibrahim Reply
what is biology
Basiru Reply
Biology deals with the study of living organisms and their interactions with each other and the environment.
Quartey
biology simply means the study of living and non-livingthings.
Shamstar
what is the meaning of ubiquitous
Hamziyatu Reply
Under what topic is this question
fred
three branches of biology
Alexander Reply

Get the best Biology course in your pocket!





Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Biology' conversation and receive update notifications?

Ask