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The first cloned animal was Dolly, a sheep who was born in 1996. The success rate of reproductive cloning at the time was very low. Dolly lived for seven years and died of respiratory complications ( [link] ). There is speculation that because the cell DNA belongs to an older individual, the age of the DNA may affect the life expectancy of a cloned individual. Since Dolly, several animals such as horses, bulls, and goats have been successfully cloned, although these individuals often exhibit facial, limb, and cardiac abnormalities. There have been attempts at producing cloned human embryos as sources of embryonic stem cells, sometimes referred to as cloning for therapeutic purposes. Therapeutic cloning produces stem cells to attempt to remedy detrimental diseases or defects (unlike reproductive cloning, which aims to reproduce an organism). Still, therapeutic cloning efforts have met with resistance because of bioethical considerations.

Art connection

To clone Dolly the sheep, a Scottish Blackface sheep was used as a cytoplasmic donor. Eggs from this sheep were extracted, and the nucleus removed. A Finn-Dorset sheep was used as the nuclear donor. Nuclei were extracted from mammary cells, and direct electric current was used to fuse the nuclear DNA with the donor egg. The egg was then allowed to divide to the blastocyst stage, in which a sphere of cells contains a cluster of cells on one side. The blastocyst was implanted in a surrogate mother, resulting in Dolly the sheep.
Dolly the sheep was the first mammal to be cloned. To create Dolly, the nucleus was removed from a donor egg cell. The nucleus from a second sheep was then introduced into the cell, which was allowed to divide to the blastocyst stage before being implanted in a surrogate mother. (credit: modification of work by "Squidonius"/Wikimedia Commons)

Do you think Dolly was a Finn-Dorset or a Scottish Blackface sheep?

Genetic engineering

Genetic engineering is the alteration of an organism’s genotype using recombinant DNA technology to modify an organism’s DNA to achieve desirable traits. The addition of foreign DNA in the form of recombinant DNA vectors generated by molecular cloning is the most common method of genetic engineering. The organism that receives the recombinant DNA is called a genetically modified organism (GMO). If the foreign DNA that is introduced comes from a different species, the host organism is called transgenic    . Bacteria, plants, and animals have been genetically modified since the early 1970s for academic, medical, agricultural, and industrial purposes. In the US, GMOs such as Roundup-ready soybeans and borer-resistant corn are part of many common processed foods.

Gene targeting

Although classical methods of studying the function of genes began with a given phenotype and determined the genetic basis of that phenotype, modern techniques allow researchers to start at the DNA sequence level and ask: "What does this gene or DNA element do?" This technique, called reverse genetics, has resulted in reversing the classic genetic methodology. This method would be similar to damaging a body part to determine its function. An insect that loses a wing cannot fly, which means that the function of the wing is flight. The classical genetic method would compare insects that cannot fly with insects that can fly, and observe that the non-flying insects have lost wings. Similarly, mutating or deleting genes provides researchers with clues about gene function. The methods used to disable gene function are collectively called gene targeting. Gene targeting is the use of recombinant DNA vectors to alter the expression of a particular gene, either by introducing mutations in a gene, or by eliminating the expression of a certain gene by deleting a part or all of the gene sequence from the genome of an organism.

Questions & Answers

in genetics which disease is also termed as the queen disease
Phinihas Reply
what are the types of cell
Teye Reply
prokaryote ,eukaryote, akaryotes
bonney
biology is the study of living organisms
bonney Reply
what are the important of cells in the body
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Is the study of living things
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Emmanuel Reply
what is diffusion
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what are the important of ecology?
Lenox
what are the importance of ecology
Foday Reply
double stranded DNA is found in which viruses?
Deborah Reply
Virusws usually dont hsve double strnaded DNA they have a single strand RNA. U should probably check them in gpogle just to be sure
Danisha
what would happen if humans were not multicellular
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ettr
Grace
sorry but no
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i haven't any things
mascuud
what is biology
Emmanuel
study of life
Dads
what are types of photosynthesis
Dads
Is there any other type of a eukaryotic cell.
Grace Reply
what is bionomial nomenclature
Rachaelda Reply
state the role of mitochondria
Rachaelda
mitochondria ia power House of the cell. it provides energy and as ATP. Cells energy currency.
Haider
The scientific method of giving short names on the basis of genius and species.
Haider
it is introduce by carlous Lennieus
Haider
it is naming of living organism where by they are given two names one generic and the other specific name
Kenneth
what is element
Kofi Reply
Structure of water molecule and it's biological significance. .....help guys
Ashly
what is the formula for chemical equetion
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Why mitochondria is called the power house of the congo the bahamas cell
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Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
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