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Although we have more contrast between our cell borders and the background, there is still a lot of noise which will need to be annihilated before edge detection can be successful. Ideally, we want to only preserve pixels which constitute a cell border and map all other pixels to a zero intensity.

Conversion to gray scale

Filtering is more easily done on a grayscale image than on a colormap image. This is due to the fact that grayscale images are intensity images with each pixel reflecting a particular intensity, rather than a combination of three colors (red, green and blue). With a grayscale image, filters such as mean and mode filters can be more appropriately applied.

Mean filtering

The first step in getting rid of unwanted pixels is to apply a mean filter. This filter will take a specified neighborhood around each pixel and set each pixel to the mean of its neighborhood. This is done with Matlab’s sliding neighborhood operation which will go through each pixel, taking its neighborhood pixels as inputs into a specified function and set each pixel as the output of the function.

In our case, the function we want to apply will take the neighboring pixels and output the mean. This mean represents the average intensity of its neighboring pixels. Thus, a lone intense (lit) pixel way out in the boondocks will be set to zero since there are no other high-intensity pixels nearby. This will effectively eliminate lone high-intensity pixels and thus decrease noise.

Following mean filtering, the image was converted to black and white. This was done by specifying a threshold intensity and setting all pixels above the threshold to 1 (white) and all pixels below to 0 (black). Why must this be done? Well, you’ll just have to read on to see… Figure 1 shows our final result in this step after converting to black and white.

Questions & Answers

Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
what school?
biomolecules are e building blocks of every organics and inorganic materials.
anyone know any internet site where one can find nanotechnology papers?
Damian Reply
sciencedirect big data base
Introduction about quantum dots in nanotechnology
Praveena Reply
what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
there is no specific books for beginners but there is book called principle of nanotechnology
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
carbon nanotubes has various application in fuel cells membrane, current research on cancer drug,and in electronics MEMS and NEMS etc
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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