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We have chosen a variety of features from literature review to explore. [1] These can be divided into two categories, binary and grayscale, which refer to the types of images that the code operates on. The binary features are based on morphological properties including cell and nucleus area and perimeter. The grayscale features describe the texture and contrast of the cells. We implemented a total of 12 features, 5 binary and 7 grayscale. All the features below were calculated for both the cell and the nucleus, except for the ratio of nucleus area to cell area.

We used 150 test images in order to extract features for our matrices. Again, our images were 250 x 250 pixels which we believed to be large enough to minimize errors from segmentation.

Binary features

Ratio of nucleus to cell area

The ratio of nucleus to cell areas can be calculated easily using a set of binary nucleus and cell images.

We calculate area by summing all of the white pixels in an image into a scalar number, and compute the ratio by dividing these numbers.

Ratio of area to perimeter

The ratio of area to perimeter is calculated for both the cell and the nucleus. Perimeter can be calculated using the MATLAB image processing function regionprops 'Perimeter', which sums continuous adjacent pixels of objects in an image.

Circularity

Circularity is calculated as a function of area and perimeter (ref paper, insert eq + variables). We calculated circularity for both the nucleus and cell. The closer the value is to 1, the more circular an object is.

Circularity formula.

Compactness

Compactness is calculated for both the cell and the nucleus. It is another measure of how circular an object is. Compactness is defined as the square root of area of the nucleus divided by the area of a circle with the same perimeter.

Compactness Equation

Grayscale features

The grayscale features are based on the gray-level co-occurrence matrix (GLCM), which can be used to extract statistical measures of texture. The GLCM is a matrix with elements p(i,j) that are equal to the number of times in the image a pixel with grayscale intensity i appears adjacent to a pixel with grayscale intensity level j. [3]

Contrast

Contrast measures the difference in grayscale intensity between adjacent pixels over the entire image. The greater the difference in intensity values, the higher the value of contrast.

Contrast equation.

Homogeneity

Homogeneity measures the distances of GLCM elements from the GLCM diagonal. Homogeneity ranges from 0 to 1. If adjacent pixels always have very similar values of grayscale intensity, the homogeneity will be close to 1.

Homogeneity equation.

Entropy

Entropy is a measure of the randomness of grayscale intensity values of pixels. Entropy is based off the grayscale histogram of the image. The histogram can be created from the GLCM by summing across the rows to find the total number of pixels p(i) for each grayscale intensity value i. [4]

Entropy equation.

Questions & Answers

where we get a research paper on Nano chemistry....?
Maira Reply
what are the products of Nano chemistry?
Maira Reply
There are lots of products of nano chemistry... Like nano coatings.....carbon fiber.. And lots of others..
learn
Even nanotechnology is pretty much all about chemistry... Its the chemistry on quantum or atomic level
learn
Google
da
no nanotechnology is also a part of physics and maths it requires angle formulas and some pressure regarding concepts
Bhagvanji
hey
Giriraj
Preparation and Applications of Nanomaterial for Drug Delivery
Hafiz Reply
revolt
da
Application of nanotechnology in medicine
what is variations in raman spectra for nanomaterials
Jyoti Reply
I only see partial conversation and what's the question here!
Crow Reply
what about nanotechnology for water purification
RAW Reply
please someone correct me if I'm wrong but I think one can use nanoparticles, specially silver nanoparticles for water treatment.
Damian
yes that's correct
Professor
I think
Professor
Nasa has use it in the 60's, copper as water purification in the moon travel.
Alexandre
nanocopper obvius
Alexandre
what is the stm
Brian Reply
is there industrial application of fullrenes. What is the method to prepare fullrene on large scale.?
Rafiq
industrial application...? mmm I think on the medical side as drug carrier, but you should go deeper on your research, I may be wrong
Damian
How we are making nano material?
LITNING Reply
what is a peer
LITNING Reply
What is meant by 'nano scale'?
LITNING Reply
What is STMs full form?
LITNING
scanning tunneling microscope
Sahil
how nano science is used for hydrophobicity
Santosh
Do u think that Graphene and Fullrene fiber can be used to make Air Plane body structure the lightest and strongest. Rafiq
Rafiq
what is differents between GO and RGO?
Mahi
what is simplest way to understand the applications of nano robots used to detect the cancer affected cell of human body.? How this robot is carried to required site of body cell.? what will be the carrier material and how can be detected that correct delivery of drug is done Rafiq
Rafiq
if virus is killing to make ARTIFICIAL DNA OF GRAPHENE FOR KILLED THE VIRUS .THIS IS OUR ASSUMPTION
Anam
analytical skills graphene is prepared to kill any type viruses .
Anam
Any one who tell me about Preparation and application of Nanomaterial for drug Delivery
Hafiz
what is Nano technology ?
Bob Reply
write examples of Nano molecule?
Bob
The nanotechnology is as new science, to scale nanometric
brayan
nanotechnology is the study, desing, synthesis, manipulation and application of materials and functional systems through control of matter at nanoscale
Damian
Is there any normative that regulates the use of silver nanoparticles?
Damian Reply
what king of growth are you checking .?
Renato
What fields keep nano created devices from performing or assimulating ? Magnetic fields ? Are do they assimilate ?
Stoney Reply
why we need to study biomolecules, molecular biology in nanotechnology?
Adin Reply
?
Kyle
yes I'm doing my masters in nanotechnology, we are being studying all these domains as well..
Adin
why?
Adin
what school?
Kyle
biomolecules are e building blocks of every organics and inorganic materials.
Joe
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Automatic white blood cell classification using svm and neural networks. OpenStax CNX. Dec 16, 2015 Download for free at http://legacy.cnx.org/content/col11924/1.5
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