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By the end of this section, you will be able to:
  • Describe the different steps in protein synthesis
  • Discuss the role of ribosomes in protein synthesis
  • Describe the genetic code and how the nucleotide sequence determines the amino acid and the protein sequence

The synthesis of proteins is one of a cell’s most energy-consuming metabolic processes. In turn, proteins account for more mass than any other component of living organisms (with the exception of water), and proteins perform a wide variety of the functions of a cell. The process of translation, or protein synthesis, involves decoding an mRNA message into a polypeptide product. Amino acids are covalently strung together in lengths ranging from approximately 50 amino acids to more than 1,000.

The protein synthesis machinery

In addition to the mRNA template, many other molecules contribute to the process of translation. The composition of each component may vary across species; for instance, ribosomes may consist of different numbers of ribosomal RNAs ( rRNA    ) and polypeptides depending on the organism. However, the general structures and functions of the protein synthesis machinery are comparable from bacteria to human cells. Translation requires the input of an mRNA template, ribosomes, tRNAs, and various enzymatic factors ( [link] ).

Illustration of the molecules involved in protein translation. A ribosome is shown with mRNA and tRNA. Amino acids are emerging to form a protein chain.
The protein synthesis machinery includes the large and small subunits of the ribosome, mRNA, and tRNA. (credit: modification of work by NIGMS, NIH)

In E. coli , there are 200,000 ribosomes present in every cell at any given time. A ribosome is a complex macromolecule composed of structural and catalytic rRNAs, and many distinct polypeptides. In eukaryotes, the nucleolus is completely specialized for the synthesis and assembly of rRNAs.

Ribosomes are located in the cytoplasm in prokaryotes and in the cytoplasm and endoplasmic reticulum of eukaryotes. Ribosomes are made up of a large and a small subunit that come together for translation. The small subunit is responsible for binding the mRNA template, whereas the large subunit sequentially binds tRNAs , a type of RNA molecule that brings amino acids to the growing chain of the polypeptide. Each mRNA molecule is simultaneously translated by many ribosomes, all synthesizing protein in the same direction.

Depending on the species, 40 to 60 types of tRNA exist in the cytoplasm. Serving as adaptors, specific tRNAs bind to sequences on the mRNA template and add the corresponding amino acid to the polypeptide chain. Therefore, tRNAs are the molecules that actually “translate” the language of RNA into the language of proteins. For each tRNA to function, it must have its specific amino acid bonded to it. In the process of tRNA “charging,” each tRNA molecule is bonded to its correct amino acid.

The genetic code

To summarize what we know to this point, the cellular process of transcription generates messenger RNA (mRNA), a mobile molecular copy of one or more genes with an alphabet of A, C, G, and uracil (U). Translation of the mRNA template converts nucleotide-based genetic information into a protein product. Protein sequences consist of 20 commonly occurring amino acids; therefore, it can be said that the protein alphabet consists of 20 letters. Each amino acid is defined by a three-nucleotide sequence called the triplet codon    . The relationship between a nucleotide codon and its corresponding amino acid is called the genetic code    .

Questions & Answers

what is ecology, ecosystem?
Nkeng Reply
what is digestive system
Lucky Reply
digestive system is the human syman system that icludes esopuges stomach o braking down of food in to useful substance to our body
samrawit
definition of biology basics
Ritu Reply
the potential energy of a molecule can be inquired by their number of?
Jesus Reply
what is the full meaning of RNA
Ayo Reply
ribose nucleic acid
Nikita
Ribonucleic acid
Jesus
discuss, describe at least three (3) methods that could be used to improve photosynthesis..
Marvel Reply
Improve the efficiency with which plants capture light Improve the efficiency by which plants turn light into energy The smart canopy concept develop crop planting schemes that increase the penetration of sunlight into lower-level leaves.
Jesus
what is osmosis
Aon Reply
movement of water molecule from higher to lower concentration through a semipereable membrene.
Dr
what of in the case of solute
Aon
osmosis is the movement of molecules from higher concentration region to lower concentration region through semi-permeable membrane.
Broad
in case of solute means that water moves from the region with lower solutes to the region with higher solute. so it is vice versa to water.
Broad
what are the hydrophilic and hydrophobic region of the plasma membrane?
Samuel Reply
hydrophilic in other word it called water loving and hydrophobic region other word is region that does not contact with water in the plasma membrane.
Broad
the phospholipids
Jesus
recognizing living things
Emmanuel Reply
Species A has 12 pairs of chromosomes and Species B has 11 pairs of chromosomes. Explain what occurs during mitosis and during meiosis in the hybrid that allows normal development and growth from zygote to adult, but causes the adults to be sterile.
Christina Reply
what is the origin of angiosperms?
Broad
unknown group of gymnosperms of triassic period.
Aditi
ouky but what about the concept of monophyletic and polyphyletic? where angiosperms is between that two concept?
Broad
Why does water move through a membrane?
Christina Reply
Explanation: Water can diffuse through the lipid bilayer even though it's polar because it's a very small molecule. Water can also pass through the cell membrane by osmosis, because of the high osmotic pressure difference between the inside and the outside the cell
Babar
How many bones are in the human skeleton
Treasure Reply
203
Oyeleke
206
Babar
it is about 270 bones at birth and decreases to 206 bones in adulthood.
Nkeng
procce of digestion of proteins a long human alimentarycanal
Carson Reply
This is accomplished by enzymes through hydrolysis.
Jesus
what are the properties of lipids?
Isiah Reply
They are: Fatty acids, fats, oils, waxes, phospholipid, glycolipids, steroids and some vitamins
Rachel
lipids are made  up of a glycerol molecule with three fatty acid molecules attached to it. 
Jesus
explain why a fresh water fish excrete ammonia
Leonard Reply
plz answer my question
Leonard
sorry i meant it has a nucleous unlike plant cells lol
Lailah
Ammonia is the end product of protein catabolism and is stored in the body of the fish in high concentrations relative to basal excretion rates. Ammonia, if allowed to accumulate, is toxic and is converted to less toxic compounds or excreted
Rachel
cartilagenous fish's are ureolitic they excrete the waste in the form of urea as there undigested food mostly forms urea in their digestive system n much of the urea iz stored in the of the cartilagenous fish's. hence they are not a edible, but boney fish's excrete ammonia as they form less amount o
Babar
i really find some stuffs hard u guys are guru's
Orji

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Source:  OpenStax, Concepts of biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11487/1.9
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