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The scientific method

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method. The scientific method was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) ( [link] ), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost all fields of study as a logical, rational problem-solving method.

Painting depicts Sir Francis Bacon in a long robe.
Sir Francis Bacon (1561–1626) is credited with being the first to define the scientific method. (credit: Paul van Somer)

The scientific process typically starts with an observation (often a problem to be solved) that leads to a question. Let’s think about a simple problem that starts with an observation and apply the scientific method to solve the problem. One Monday morning, a student arrives at class and quickly discovers that the classroom is too warm. That is an observation that also describes a problem: the classroom is too warm. The student then asks a question: “Why is the classroom so warm?”

Proposing a hypothesis

Recall that a hypothesis is a suggested explanation that can be tested. To solve a problem, several hypotheses may be proposed. For example, one hypothesis might be, “The classroom is warm because no one turned on the air conditioning.” But there could be other responses to the question, and therefore other hypotheses may be proposed. A second hypothesis might be, “The classroom is warm because there is a power failure, and so the air conditioning doesn’t work.”

Once a hypothesis has been selected, the student can make a prediction. A prediction is similar to a hypothesis but it typically has the format “If . . . then . . . .” For example, the prediction for the first hypothesis might be, “ If the student turns on the air conditioning, then the classroom will no longer be too warm.”

Testing a hypothesis

A valid hypothesis must be testable. It should also be falsifiable    , meaning that it can be disproven by experimental results. Importantly, science does not claim to “prove” anything because scientific understandings are always subject to modification with further information. This step—openness to disproving ideas—is what distinguishes sciences from non-sciences. The presence of the supernatural, for instance, is neither testable nor falsifiable. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. Each experiment will have one or more variables and one or more controls. A variable    is any part of the experiment that can vary or change during the experiment. The control group contains every feature of the experimental group except it is not given the manipulation that is hypothesized about. Therefore, if the results of the experimental group differ from the control group, the difference must be due to the hypothesized manipulation, rather than some outside factor. Look for the variables and controls in the examples that follow. To test the first hypothesis, the student would find out if the air conditioning is on. If the air conditioning is turned on but does not work, there should be another reason, and this hypothesis should be rejected. To test the second hypothesis, the student could check if the lights in the classroom are functional. If so, there is no power failure and this hypothesis should be rejected. Each hypothesis should be tested by carrying out appropriate experiments. Be aware that rejecting one hypothesis does not determine whether or not the other hypotheses can be accepted; it simply eliminates one hypothesis that is not valid ( [link] ). Using the scientific method, the hypotheses that are inconsistent with experimental data are rejected.

Questions & Answers

Complex traits such as height result from 
Ruben Reply
what is the difference between chloroplasts and mitochondria
Nkalubo Reply
chloroplast in plants and bacterial cell ; mitochondria in animal cells
aung
Diagram of a living cell
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what is cell
Sule
A cell is the smallest basic unit of life.
John
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Ogochukwu Reply
this is da study of living and non-living thing in an eco-system
Nutty
it is the study of living and non living organism in the ecology
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Winner
why do plants store carbohydrates in form of starch and not glucose?
Nutty Reply
Describe the structure of starch?
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wat is diffusion
Winner
water is life!.. Discuss?
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why do plants store carbohydrates in form if starch not glucose!
Nutty
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The surgical removal of d spermduct
Eniola
What is Staining?
Fazal Reply
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PEACE Reply
Biology is the study of life
Tijani
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Ysabella Reply
biology is a study of living things
PEACE
Biology is a diverse branch of science that deals with mostly living things
Emmanuel
yes
Swapnil
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Swapnil
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SUZAN Reply
what is the mechanism of cellular respiration
Rita Reply
what is enzyme
garry Reply
They are organic catalysts that alter the rate of chemical reactions in the body.
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Sarni
Enzymes are forms of chemicals that are specialized in their own areas.(eg digestion of food)
Emmanuel
Enzymes are organic catalysts
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Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
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