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The first cloned animal was Dolly, a sheep who was born in 1996. The success rate of reproductive cloning at the time was very low. Dolly lived for seven years and died of respiratory complications ( [link] ). There is speculation that because the cell DNA belongs to an older individual, the age of the DNA may affect the life expectancy of a cloned individual. Since Dolly, several animals such as horses, bulls, and goats have been successfully cloned, although these individuals often exhibit facial, limb, and cardiac abnormalities. There have been attempts at producing cloned human embryos as sources of embryonic stem cells, sometimes referred to as cloning for therapeutic purposes. Therapeutic cloning produces stem cells to attempt to remedy detrimental diseases or defects (unlike reproductive cloning, which aims to reproduce an organism). Still, therapeutic cloning efforts have met with resistance because of bioethical considerations.

Art connection

To clone Dolly the sheep, a Scottish Blackface sheep was used as a cytoplasmic donor. Eggs from this sheep were extracted, and the nucleus removed. A Finn-Dorset sheep was used as the nuclear donor. Nuclei were extracted from mammary cells, and direct electric current was used to fuse the nuclear DNA with the donor egg. The egg was then allowed to divide to the blastocyst stage, in which a sphere of cells contains a cluster of cells on one side. The blastocyst was implanted in a surrogate mother, resulting in Dolly the sheep.
Dolly the sheep was the first mammal to be cloned. To create Dolly, the nucleus was removed from a donor egg cell. The nucleus from a second sheep was then introduced into the cell, which was allowed to divide to the blastocyst stage before being implanted in a surrogate mother. (credit: modification of work by "Squidonius"/Wikimedia Commons)

Do you think Dolly was a Finn-Dorset or a Scottish Blackface sheep?

Genetic engineering

Genetic engineering is the alteration of an organism’s genotype using recombinant DNA technology to modify an organism’s DNA to achieve desirable traits. The addition of foreign DNA in the form of recombinant DNA vectors generated by molecular cloning is the most common method of genetic engineering. The organism that receives the recombinant DNA is called a genetically modified organism (GMO). If the foreign DNA that is introduced comes from a different species, the host organism is called transgenic    . Bacteria, plants, and animals have been genetically modified since the early 1970s for academic, medical, agricultural, and industrial purposes. In the US, GMOs such as Roundup-ready soybeans and borer-resistant corn are part of many common processed foods.

Gene targeting

Although classical methods of studying the function of genes began with a given phenotype and determined the genetic basis of that phenotype, modern techniques allow researchers to start at the DNA sequence level and ask: "What does this gene or DNA element do?" This technique, called reverse genetics, has resulted in reversing the classic genetic methodology. This method would be similar to damaging a body part to determine its function. An insect that loses a wing cannot fly, which means that the function of the wing is flight. The classical genetic method would compare insects that cannot fly with insects that can fly, and observe that the non-flying insects have lost wings. Similarly, mutating or deleting genes provides researchers with clues about gene function. The methods used to disable gene function are collectively called gene targeting. Gene targeting is the use of recombinant DNA vectors to alter the expression of a particular gene, either by introducing mutations in a gene, or by eliminating the expression of a certain gene by deleting a part or all of the gene sequence from the genome of an organism.

Questions & Answers

what structure help root hair cells to take up water.
Jackson Reply
which two part of a plant cell are affected when the is immersed in sucrose solution?
Jackson
which two part of a plant cell are affected when the is immersed in sucrose solution?
Jackson
the xylem cells absorbs water and mineral salt from the soil to all part of the plant.
Nana
And the phloem cell take up food prepared from the leaves to all part of the plant
Nana
what is genetic engineering
Mavis Reply
what are the three main type of ecosystem
Mavis
biosphere,
Bigenis
biosphere
Adeniyi
what is mitochondria
Might Reply
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Abanke Reply
what is genetic engineering
Eveline Reply
what is the meaning of term mitosis
Lwitiko Reply
outline the significance of mitosis to organisms
Lwitiko
significant of meiosis are to increase in variation and leads to the formation of haploid gamete
Eveline
thanks
Abanke
name the resources to be conserved
Oreva Reply
define natural resources
Oreva
name the agencies responsible for the conservation of natural resources
Oreva
land,water,forests,
Kiiza
nema,uwa
Kiiza
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Peace
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Erika
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garnhial
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garnhial
explain the importance of carbon dioxide in the body
Kiiza Reply
how does that works
SAMUEL
diagram of Prokaryotic cells
Magreth Reply
where is it?
Yazi
ʜɪ
Malikie
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Malikie
where is the diagram?
Yazi
waiting.....
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Joelia
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Paul
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Tafadzwa
Define the term Biology element atom
James
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Rons
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Israel
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Reine
what is a cell of a bacteria called
Sahfe Reply
what is genotype
James
genotype: this is the combination of alleles an organism has for a given characteristic..
Cosmo
what is maiotic
Gabriel Reply
what is sexual reproduction
Gabriel
is system which usually happens to sexual things
GABRIEL
what are the effects of concentration gradient in the uptake of water by plant's
Harleen Reply
ls it ok if you have sex during pregnancy
Kags Reply
no
Bernard
yes!!!
Yazi
Yes
Babie
hello
Cabdi
Babie Maseuse .. Hello .. how are you ?
kf
yes it make the baby's strength stay longer
REAL
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Babie
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Lamin
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Lamin
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Lamin
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Lamin
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Tony
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Lamin
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Nyakato
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george
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Lamin
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george
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Cosmo
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Joelia
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Lamin
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Joelia
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Lamin
yes
James
What is the meaning of organ
Ronald Reply
tissue combined to form organ
zameer
join
Elishs
orga- these are tissues join together to perform a specific functions.
Elishs
then what are tissues
Kags
group of cells working together to perform a particular function
Harleen
tissue are group of cells put together to perform a certain goal
Bernard
Organs are group of tissues working together to perform a specific function
Peter
What will happen when read blood cell placed in 0.5 % Nacl solution
zameer Reply
it will shrink and collapse eventually since the concentration of the solution is higher than the cell content
Peter

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Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
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