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By the end of this section, you will be able to:
  • Describe the overall result in terms of molecules produced in the breakdown of glucose by glycolysis
  • Compare the output of glycolysis in terms of ATP molecules and NADH molecules produced

You have read that nearly all of the energy used by living cells comes to them in the bonds of the sugar, glucose. Glycolysis is the first step in the breakdown of glucose to extract energy for cellular metabolism. Nearly all living organisms carry out glycolysis as part of their metabolism. The process does not use oxygen and is therefore anaerobic    . Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport in which the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These transporters assist in the facilitated diffusion of glucose.

Glycolysis begins with the six carbon ring-shaped structure of a single glucose molecule and ends with two molecules of a three-carbon sugar called pyruvate    . Glycolysis consists of two distinct phases. The first part of the glycolysis pathway traps the glucose molecule in the cell and uses energy to modify it so that the six-carbon sugar molecule can be split evenly into the two three-carbon molecules. The second part of glycolysis extracts energy from the molecules and stores it in the form of ATP and NADH, the reduced form of NAD.

First half of glycolysis (energy-requiring steps)

Step 1. The first step in glycolysis ( [link] ) is catalyzed by hexokinase, an enzyme with broad specificity that catalyzes the phosphorylation of six-carbon sugars. Hexokinase phosphorylates glucose using ATP as the source of the phosphate, producing glucose-6-phosphate, a more reactive form of glucose. This reaction prevents the phosphorylated glucose molecule from continuing to interact with the GLUT proteins, and it can no longer leave the cell because the negatively charged phosphate will not allow it to cross the hydrophobic interior of the plasma membrane.

Step 2. In the second step of glycolysis, an isomerase converts glucose-6-phosphate into one of its isomers, fructose-6-phosphate. An isomerase    is an enzyme that catalyzes the conversion of a molecule into one of its isomers. (This change from phosphoglucose to phosphofructose allows the eventual split of the sugar into two three-carbon molecules.).

Step 3. The third step is the phosphorylation of fructose-6-phosphate, catalyzed by the enzyme phosphofructokinase. A second ATP molecule donates a high-energy phosphate to fructose-6-phosphate, producing fructose-1,6- bi sphosphate. In this pathway, phosphofructokinase is a rate-limiting enzyme. It is active when the concentration of ADP is high; it is less active when ADP levels are low and the concentration of ATP is high. Thus, if there is “sufficient” ATP in the system, the pathway slows down. This is a type of end product inhibition, since ATP is the end product of glucose catabolism.

Questions & Answers

prove starch in a mango leaf
Ruth Reply
prove starch in a leaf
Ruth
draw and name the plant cell
Nalongo
wat is classification
Nalongo
levels of classification
Nalongo
classification of what
Ezenwa
Identify the caste of termites or Honey bees
Mensah Reply
Soldier Winged reproductive Worker King and queen
Franklina
what is biology
Jackson Reply
biology is a study of living organism
Brilliant
what is the myelin sheath?
description on how an enzyme such as pepsin breaks down a substrate
linister Reply
what is biology
Kanzy Reply
study of life
Tufail
the study of life..meaning studying all aspects of life in humans,animals and everything inside the global
Nalukui
study of life
Memory
is the branch of science which deal with the study of living and non living things
David
Is the study of living organisms or living things
Franklina
draw the taxonomic hierarchy of classification
KC Reply
Kingdom Class Order Family Genus Species
Franklina
explain the following bush burning, deforestation, over grazing
Thomas Reply
what is biodiversity
Tracy
No idea
nana
Please what are alleles
nana
Deforestation: this is the cutting down of trees without planting new ones
Brilliant
over grazing is the continuously planting of crops in one particular land.
Friday
what is afforestation
Friday
Bush burning is the removal of the natural vegetation cover, that protects the soil surface through the use of fire. Deforestation is the actor process of establishing a forest especially on land not previously forested.
Franklina
Over grazing is the practice of grazing toi many livestock for too long a period on a land unable to recover its vegetation.
Franklina
what is physiology
Joseph Reply
the study of physical things
Nalukui
dealing with those things which you can see by your neck eyes
Odwa
Further explanation please
nana
the study of things tt interact with time,energy
David
Is the scientific study of chemistry and physics of the structure of the body
Franklina
can a lactating mother get pregnant
Elias Reply
what is cell
Deng Reply
the basic building blocks of all living matter...
Izzati
cell is a basic unit of life
MAI
building blocks ie cells not a cell there4 a cell is the basic unit of biotic things
David
Cell is a basic unit of life
Malekhotla
A cell is the basic structural and functional unit of life
Franklina
what's microscope
Emmanuel Reply
is any instrument that use to wiew or to see the small object that you can't see with our naked eye
MR
is any instrument used to magnifie small object
Memory
Is an instrument that produces an enlarged image of an object or specimen that is too small to be seen with our naked eye
Franklina
what is the function of the spinal cord
Gaone Reply
For responsible of movement and sensation
Franklina
A cell is the structural and fictional unit of life
Evelyn Reply
what is tidal volume
Erick
tidal volume is the volume of each breath measured during inspiration or expiration or averged for the entire respiratory cycle
Gaone
Give function of the long strands of slippery or jelly like substance in eggs of the amphibians in water ?
Erick
the long strands of slippery or jelly like substance him eggs of amphibians in water helps in keeping the eggs moist and protection from predators
Gaone
sorry not him in
Gaone
what's is microscope
Emmanuel
How do bacteria useful?
Bisirikirwa
how can plant feed other animals
Nana Reply
After the animals has death and decayed then the plant used the nutrients to manufacture there food.
Yusuf
Plants and animals depends on each other. Meaning, plants help animals with greens and fruits or vegetables while animals provides nutrients when dead and decayed. Therefore,as you see the food web and food chain, you'll have more.
Jairenaririko

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Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
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