<< Chapter < Page Chapter >> Page >
Part A shows antibody neutralization. Antibodies coat the surface of a virus or toxic protein, such as the diphtheria toxin, and prevent them from binding to their target. Part B shows opsonization, a process by which a pathogen coated with antigens is consumed by a macrophage or neutrophil. Part C shows complement activation. Antibodies attached to the surface of a pathogen cell activate the complement system. Pores are formed in the cell membrane, destroying the cell.
Antibodies may inhibit infection by (a) preventing the antigen from binding its target, (b) tagging a pathogen for destruction by macrophages or neutrophils, or (c) activating the complement cascade.

Antibodies also mark pathogens for destruction by phagocytic cells, such as macrophages or neutrophils, because phagocytic cells are highly attracted to macromolecules complexed with antibodies. Phagocytic enhancement by antibodies is called opsonization. In a process called complement fixation, IgM and IgG in serum bind to antigens and provide docking sites onto which sequential complement proteins can bind. The combination of antibodies and complement enhances opsonization even further and promotes rapid clearing of pathogens.

Affinity, avidity, and cross reactivity

Not all antibodies bind with the same strength, specificity, and stability. In fact, antibodies exhibit different affinities (attraction) depending on the molecular complementarity between antigen and antibody molecules, as illustrated in [link] . An antibody with a higher affinity for a particular antigen would bind more strongly and stably, and thus would be expected to present a more challenging defense against the pathogen corresponding to the specific antigen.

Part A compares affinity and avidity. Affinity refers to the strength of a single antibody–antigen interaction. Each IgG antigen-binding site typically has high affinity for its target. Avidity refers to the strength of all interactions combined, IgM typically has low affinity antigen binding sites, but there are ten of them so avidity is high. Part B describes cross reactivity, a situation in which an antibody reacts with two different epitopes.
(a) Affinity refers to the strength of single interaction between antigen and antibody, while avidity refers to the strength of all interactions combined. (b) An antibody may cross react with different epitopes.

The term avidity    describes binding by antibody classes that are secreted as joined, multivalent structures (such as IgM and IgA). Although avidity measures the strength of binding, just as affinity does, the avidity is not simply the sum of the affinities of the antibodies in a multimeric structure. The avidity depends on the number of identical binding sites on the antigen being detected, as well as other physical and chemical factors. Typically, multimeric antibodies, such as pentameric IgM, are classified as having lower affinity than monomeric antibodies, but high avidity. Essentially, the fact that multimeric antibodies can bind many antigens simultaneously balances their slightly lower binding strength for each antibody/antigen interaction.

Antibodies secreted after binding to one epitope on an antigen may exhibit cross reactivity for the same or similar epitopes on different antigens. Because an epitope corresponds to such a small region (the surface area of about four to six amino acids), it is possible for different macromolecules to exhibit the same molecular identities and orientations over short regions. Cross reactivity describes when an antibody binds not to the antigen that elicited its synthesis and secretion, but to a different antigen.

Cross reactivity can be beneficial if an individual develops immunity to several related pathogens despite having only been exposed to or vaccinated against one of them. For instance, antibody cross reactivity may occur against the similar surface structures of various Gram-negative bacteria. Conversely, antibodies raised against pathogenic molecular components that resemble self molecules may incorrectly mark host cells for destruction and cause autoimmune damage. Patients who develop systemic lupus erythematosus (SLE) commonly exhibit antibodies that react with their own DNA. These antibodies may have been initially raised against the nucleic acid of microorganisms but later cross-reacted with self-antigens. This phenomenon is also called molecular mimicry.

Antibodies of the mucosal immune system

Antibodies synthesized by the mucosal immune system include IgA and IgM. Activated B cells differentiate into mucosal plasma cells that synthesize and secrete dimeric IgA, and to a lesser extent, pentameric IgM. Secreted IgA is abundant in tears, saliva, breast milk, and in secretions of the gastrointestinal and respiratory tracts. Antibody secretion results in a local humoral response at epithelial surfaces and prevents infection of the mucosa by binding and neutralizing pathogens.

Section summary

Antibodies (immunoglobulins) are the molecules secreted from plasma cells that mediate the humoral immune response. There are five antibody classes; an antibody's class determines its mechanism of action and production site but does not control its binding specificity. Antibodies bind antigens via variable domains and can either neutralize pathogens or mark them for phagocytosis or activate the complement cascade.

Questions & Answers

what is genetics
Musa Reply
what is meiosis?
Musa
list three vertebrate animal and three invertebrate animal
Katie Reply
lizard, goat, frog ,earthworm, housefly, tapeworm
idoko
what is meant by radial symmetry?
Boadi
what is meaning of polysaccharide
mark Reply
water as a function in cell for inorganic substances
chisom Reply
hi john....wat about for mineral salts, its function in a cell and structure
chisom
hi chisom which topic is this
Ezeh
this biology is for which grade?
Janet Reply
A1
Boadi
state any one specialized animal cell and it's function
Pascal Reply
sperm cell: Fertilization
Tshering
Can DNA and RNA be praticalized
Oyewale Reply
you can make models of them and show how they replicate
Oswald
what is dentition
Tisa Reply
Study of teeths and their functions
Stanisla
what are the function
Tisa
it's not teeths. teeth is plural as well as singular noun. the function of the teeth is to cut and grind food for easier swallowing and for increasing the surface area if the fish as the substrate in which digestive enzymes work
Oswald
dentition is not the study of teeth but the arrangement of teeth in the mouth of species or individuals
Oswald
what are the two types of teeth
Tisa
Function of probosis
Adeola Reply
why do we study biology?
SYDNEY Reply
where biology began
Alhaji
What are chromesome
Talkmore Reply
condensed DNA wrapped with histone
Elias
chromosomes are gins that we take from our parents.when mom get pregnant the baby inside is made up of 23 chromosomes from his dad and 23 chromosomes from his mom.so a baby is made up of totally 46 chromosomes
ezera
chromosome are rod or thread shape body located in the nucleus of a cell.
Alhaji
what are chromosomes
Persiviour
what is chromosome?
Royd Reply
How can a person who has been in the vascular disease repairs his or her DNA
Rabson Reply
i need its explanation sir..pls provid na...🙁
Shaf Reply
Of wat
John
explanation for wat
Christian
Of war na so dat I can provide
John
Is d process which food is converted into substance dat can be utilize by d body
John
the meaning of disgestion
Charity Reply

Get the best Biology course in your pocket!





Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Biology' conversation and receive update notifications?

Ask